Cloned (Comment) | Organism |
---|---|
gene FAD7, DNA and amino acid sequence determination and analysis, genotyping, expression of T286 enzyme mutants | Chlamydomonas reinhardtii |
Protein Variants | Comment | Organism |
---|---|---|
additional information | mutation of Thr286 may directly affect the structure of the transmembrane domain of the enzyme | Chlamydomonas reinhardtii |
T286C | site-directed mutagenesis, omega3-fatty acid deficiency mutant | Chlamydomonas reinhardtii |
T286G | site-directed mutagenesis, omega3-fatty acid deficiency mutant | Chlamydomonas reinhardtii |
T286H | site-directed mutagenesis, omega3-fatty acid deficiency mutant | Chlamydomonas reinhardtii |
T286N | naturally occuring mutation causing omega3-fatty acid deficiency in strain CC-620, that can be recovered by overexpression of the wild-type enzyme from strain CC-125 | Chlamydomonas reinhardtii |
T286S | site-directed mutagenesis, the mutant shows partially reduced enzyme activity | Chlamydomonas reinhardtii |
T286Y | site-directed mutagenesis, omega3-fatty acid deficiency mutant | Chlamydomonas reinhardtii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Chlamydomonas reinhardtii | 16020 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlamydomonas reinhardtii | A8HMC4 | gene fad7 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | the phosphorylation target site is Thr286 | Chlamydomonas reinhardtii |
Synonyms | Comment | Organism |
---|---|---|
FAD7 | - |
Chlamydomonas reinhardtii |
fatty acid desaturase 7 | - |
Chlamydomonas reinhardtii |
General Information | Comment | Organism |
---|---|---|
malfunction | the omega-3 fatty acids hexadecatrienoic acid (C16:3n3), hexadecatetraenoic acid (C16:4),and alpha-linolenic acid (C18:3n3) are not detected in the CC-620 strain, phenotype and fatty acid composition of the omega-3 fatty acid deficiency in strain CC-620 due to the missense mutation detected in gene CrFAD7, complementation by the wild-type enzyme, overview | Chlamydomonas reinhardtii |
physiological function | Thr residue located in the fourth transmembrane domain of fatty acid desaturase 7 (FAD7) that is essential for the biosynthesis of omega-3 fatty acids in Chlamydomonas reinhardtii, Thr286 is essential for the maintaining the catalytic structure of the enzyme | Chlamydomonas reinhardtii |