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Literature summary for 1.14.19.3 extracted from

  • Okayasu, T.; Nagao, M.; Ishibashi, T.; Imai, Y.
    Purification and partial characterization of linoleoyl-CoA desaturase from rat liver microsomes (1981), Arch. Biochem. Biophys., 206, 21-28.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
NADH-cytochrome b5 reductase EC 1.6.2.2, absolutely essential for DELTA6-desaturation Rattus norvegicus
NADPH-cytochrome b5 reductase EC 1.6.2.2, absolutely essential for DELTA6-desaturation Rattus norvegicus
O2 absolutely essential for DELTA6-desaturation Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
bathophenanthroline sulfonate mild inhibition Rattus norvegicus
beta-mercaptoethanol mild inhibition Rattus norvegicus
dithiothreitol mild inhibition Rattus norvegicus
iron chelators
-
Rattus norvegicus
KCN
-
Rattus norvegicus
N-ethylmaleimide only little effect Rattus norvegicus
p-chloromercuribenzene sulfonate
-
Rattus norvegicus
Tiron
-
Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.045
-
linoleoyl-CoA
-
Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome membrane Rattus norvegicus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ DELTA6-desaturase contains 15.1 nmol iron/mg protein Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000 68000 gel filtration Rattus norvegicus
66000
-
SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
linoleic acid + AH2 + O2 Rattus norvegicus
-
gamma-linolenic acid + A + H2O
-
?
linoleic acid + AH2 + O2 Rattus norvegicus Wistar
-
gamma-linolenic acid + A + H2O
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-
Rattus norvegicus Wistar
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phospholipoprotein 20-40 M phospholipid per mol of protein Rattus norvegicus

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0344
-
-
Rattus norvegicus

Storage Stability

Storage Stability Organism
-70°C, stored under nitrogen the enzyme is unstable, loses 80% of its activity after repeated freezing and thawing Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
linoleic acid + AH2 + O2
-
Rattus norvegicus gamma-linolenic acid + A + H2O
-
?
linoleic acid + AH2 + O2
-
Rattus norvegicus Wistar gamma-linolenic acid + A + H2O
-
?
linoleoyl-CoA + AH2 + O2
-
Rattus norvegicus gamma-linolenoyl-CoA + A + 2 H2O
-
?
linoleoyl-CoA + AH2 + O2
-
Rattus norvegicus Wistar gamma-linolenoyl-CoA + A + 2 H2O
-
?
octadeca-9,12-dienoic acid + AH2 + O2
-
Rattus norvegicus gamma-linolenic acid + A + H2O
-
?
octadeca-9,12-dienoic acid + AH2 + O2
-
Rattus norvegicus Wistar gamma-linolenic acid + A + H2O
-
?

Subunits

Subunits Comment Organism
monomer 1 * 66000, SDS-PAGE Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
6 9
-
Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
cytochrome b5 absolutely essential for DELTA6-desaturation Rattus norvegicus
linoleoyl-CoA absolutely essential for DELTA6-desaturation Rattus norvegicus
NADH absolutely essential for DELTA6-desaturation Rattus norvegicus
NADPH absolutely essential for DELTA6-desaturation Rattus norvegicus