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Literature summary for 1.14.19.26 extracted from
- DELTA6 Hexadecenoic acid is synthesized by the activity of a soluble DELTA6 palmitoyl-acyl carrier protein desaturase in Thunbergia alata endosperm (1994), J. Biol. Chem., 269, 27519-27526.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Thunbergia alata |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| H2O2 | loss of most of the desaturase activity | Thunbergia alata |  |
| KCN | 1 mM, loss of most of the desaturase activity | Thunbergia alata | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| palmitoyl-[acyl-carrier protein] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ | Thunbergia alata | no desaturation activity with palmitoyl-CoA ester | (Z)-hexadec-6-enoyl-[acyl-carrier protein] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O | DELTA6-hexadecenoyl-[acyl-carrier protein] i.e. sapienoyl-[acyl-carrier-protein] i.e. (Z)-hexadec-6-enoyl-[acyl-carrier protein] | ? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thunbergia alata | Q41510 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| endosperm | - |
Thunbergia alata | - |
| seed | - |
Thunbergia alata | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| myristoyl-[acyl-carrier protein] + 2 reduced ferredoxin [iron-sulfur] cluster+ O2 + 2 H+ | - |
Thunbergia alata | (Z)-tetradec-6-enoyl-[acyl-carrier protein] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O | - |
? | |
| palmitoyl-[acyl-carrier protein] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ | no desaturation activity with palmitoyl-CoA ester | Thunbergia alata | (Z)-hexadec-6-enoyl-[acyl-carrier protein] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O | DELTA6-hexadecenoyl-[acyl-carrier protein] i.e. sapienoyl-[acyl-carrier-protein] i.e. (Z)-hexadec-6-enoyl-[acyl-carrier protein] | ? | |
| palmitoyl-[acyl-carrier protein] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ | no desaturation activity with palmitoyl-CoA. DELTA6 desaturase activity is approximately 7-fold higher using palmitoyl-[acyl-carrier protein] as substrate rather than either myristoyl-[acyl-carrier protein] or stearoyl-[acyl-carrier protein] | Thunbergia alata | (Z)-hexadec-6-enoyl-[acyl-carrier protein] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O | DELTA6-hexadecenoyl-[acyl-carrier protein] i.e. sapienoyl-[acyl-carrier-protein] i.e. (Z)-hexadec-6-enoyl-[acyl-carrier protein] | ? | |
| stearoyl-[acyl-carrier protein] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ | - |
Thunbergia alata | (Z)-octadec-6-enoyl-[acyl-carrier protein] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DELTA6 16:0-ACP desaturase | - |
Thunbergia alata |
| DELTA6 palmitoyl-ACP desaturase | - |
Thunbergia alata |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Thunbergia alata |
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