Inhibitors | Comment | Organism | Structure |
---|---|---|---|
azide | at high concentrations | Psychrobacter urativorans | |
CN- | at high concentrations | Psychrobacter urativorans | |
glutathione | reduced, not marked | Psychrobacter urativorans | |
L-cysteine | not marked | Psychrobacter urativorans | |
o-phenanthroline | partially inhibitory | Psychrobacter urativorans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | bound | Psychrobacter urativorans | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | 20-25% stimulation | Psychrobacter urativorans | |
additional information | no special ion requirement | Psychrobacter urativorans | |
Zn2+ | 20-25% stimulation | Psychrobacter urativorans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Psychrobacter urativorans | biosynthesis of unsaturated fatty acids. Possible substrates: acyl-CoA, acyl-acyl-carrier and acyl chains of phospholipids. The substrate in vivo is saturated phospholipid | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Psychrobacter urativorans | - |
ATCC 15174 | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
stearoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ = oleoyl-CoA + 2 ferricytochrome b5 + 2 H2O | incorporation of fatty acid into phospholipid. Enzyme may contain an essential thiol group. Endogenous saturated phospholipid is desaturated rapidly in all experiments in contrast with exogenous saturated phospholipid, which generally is not desaturated | Psychrobacter urativorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | not acyl-CoA as substrate but phospholipid | Psychrobacter urativorans | ? | - |
? | |
additional information | biosynthesis of unsaturated fatty acids. Possible substrates: acyl-CoA, acyl-acyl-carrier and acyl chains of phospholipids. The substrate in vivo is saturated phospholipid | Psychrobacter urativorans | ? | - |
? | |
stearate + AH2 + O2 | - |
Psychrobacter urativorans | oleate + A + 2 H2O | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
21 | - |
assay at | Psychrobacter urativorans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.7 | - |
but assay at pH 7.4, to standardize the experiments in vitro with those carried out with intact bacteria | Psychrobacter urativorans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | requires some other form of reduced cofactor than a reduced nicotinamide nucleotide, no special cofactor requirement | Psychrobacter urativorans |