Application | Comment | Organism |
---|---|---|
synthesis | coexpression of fatty acid hydroxylase FAH and diacylglycerol acyl transferase DGAT1 in Pichia pastoris produces higher lipid contents and ricinoleic acid levels than expression of FAH alone. Coexpression in a mutant haploid strain defective in the DELTA12 desaturase activity results in a higher level of ricinoleic acid than that in the diploid strain. The ricinoleic acid produced is mainly distributed in the neutral lipid fractions, particularly the free fatty acid form, but with little in the polar lipids | Claviceps purpurea |
Cloned (Comment) | Organism |
---|---|
expression in Pichia pastoris | Claviceps purpurea |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Claviceps purpurea | B4YQU1 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
oleic acid + 2 ferrocytochrome b5 + O2 + 2 H+ | - |
Claviceps purpurea | ricinoleic acid + 2 ferricytochrome b5 + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
FAH | - |
Claviceps purpurea |