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Literature summary for 1.14.18.3 extracted from

  • Gvozdev, R.; Tukhvatullin, I.; Tumanova, L.
    Purification and properties of membrane-bound methane hydroxylase from Methylococcus capsulatus (Strain M) (2008), Biol. Bull., 35, 161-169.
No PubMed abstract available
Search for more literature for 1.14.18.3

Activating Compound

Activating Compound Comment Organism Structure
additional information the active center of pMH is located in the beta-subunit Methylococcus capsulatus

Inhibitors

Inhibitors Comment Organism Structure
Acetylene is a competitive inhibitor of pMH, interacts with its substrate-binding center Methylococcus capsulatus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Methylococcus capsulatus 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ pMH contains 2-4 atoms of copper per a minimum molecular weight of 99 kDa Methylococcus capsulatus
Iron pMH contains 1 or 2 atoms of nonheme iron Methylococcus capsulatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
 pMH, 1 * 47000, + 1 * 27000 + 1 * 25000, alphabetagamma-subunit, SDS-PAGE Methylococcus capsulatus
27000
-
 pMH, 1 * 47000, + 1 * 27000 + 1 * 25000, alphabetagamma-subunit, SDS-PAGE Methylococcus capsulatus
47000
-
 pMH, 1 * 47000, + 1 * 27000 + 1 * 25000, alphabetagamma-subunit, SDS-PAGE Methylococcus capsulatus
99000
-
-
 Methylococcus capsulatus

Organism

Organism UniProt Comment Textmining
Methylococcus capsulatus
-
-
-
Methylococcus capsulatus M
-
-
-

Purification (Commentary)

Purification (Comment) Organism
particulate form of methane hydroxylase (pMH) obtained by ion exchange and hydrophobic chromatography Methylococcus capsulatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methane + reduced acceptor + H* + O2
-
 Methylococcus capsulatus methanol + acceptor + H2O
-
 ?
methane + reduced acceptor + H* + O2
-
 Methylococcus capsulatus M methanol + acceptor + H2O
-
 ?
Mn2+ + H2O2
-
 Methylococcus capsulatus ?
-
 ?
Mn2+ + H2O2
-
 Methylococcus capsulatus M ?
-
 ?

Subunits

Subunits Comment Organism
heterotrimer pMH, 1 * 47000, + 1 * 27000 + 1 * 25000, alphabetagamma-subunit, SDS-PAGE Methylococcus capsulatus

Synonyms

Synonyms Comment Organism
membrane-bound methane monooxygenase
-
 Methylococcus capsulatus
methane hydroxylase
-
 Methylococcus capsulatus
PMH
-
 Methylococcus capsulatus
pMMO
-
 Methylococcus capsulatus