Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | pMMO, optimal at 0.3 mM | Methylococcus capsulatus | |
Fe3+ | pMMO, optimal at 5.0 mM | Methylococcus capsulatus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-Heptyl-4-hydroxyquinoline-N-oxide | pMMO, at 0.05 mM | Methylococcus capsulatus | |
Acetylene | pMMO | Methylococcus capsulatus | |
Cu+ | pMMO | Methylococcus capsulatus | |
cysteine | pMMO | Methylococcus capsulatus | |
Fe2+ | slightly, membrane-bound enzyme form | Methylococcus capsulatus | |
KCN | pMMO | Methylococcus capsulatus | |
Myxothiazol | pMMO, suicide substrate | Methylococcus capsulatus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | cytoplasmatic, soluble enzyme form termed sMMO | Methylococcus capsulatus | 5737 | - |
membrane | membrane-bound particulate enzyme form termed pMMO | Methylococcus capsulatus | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 14.5 atoms per molecule of enzyme pMMO, type II copper centre | Methylococcus capsulatus | |
Iron | 2.5 atoms per enzyme molecule of pMMO | Methylococcus capsulatus | |
Mn2+ | pMMO, low content | Methylococcus capsulatus | |
Mo2+ | pMMO, low content | Methylococcus capsulatus | |
additional information | copper-induced iron-uptake | Methylococcus capsulatus | |
Zn2+ | pMMO | Methylococcus capsulatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
1 * 47000 + 1 * 27000 + 1 * 25000, pMMO, mass spectrometry and SDS-PAGE | Methylococcus capsulatus |
27000 | - |
1 * 47000 + 1 * 27000 + 1 * 25000, pMMO, mass spectrometry and SDS-PAGE | Methylococcus capsulatus |
47000 | - |
1 * 47000 + 1 * 27000 + 1 * 25000, pMMO, mass spectrometry and SDS-PAGE | Methylococcus capsulatus |
99000 | - |
pMMO, mass spectrometry | Methylococcus capsulatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylococcus capsulatus | - |
Bath | - |
Methylococcus capsulatus | - |
enzyme form pMMO is copper-inducible | - |
Methylococcus capsulatus Bath | - |
Bath | - |
Purification (Comment) | Organism |
---|---|
pMMO | Methylococcus capsulatus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
methane + quinol + O2 = methanol + quinone + H2O | mechanism of pMMO | Methylococcus capsulatus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.011 | - |
purified enzyme form pMMO | Methylococcus capsulatus |
0.0605 | - |
whole cells, substrate formate | Methylococcus capsulatus |
Storage Stability | Organism |
---|---|
4°C, reducing argon or nitrogen atmosphere, no loss of activity after 1 week | Methylococcus capsulatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methane + reduced acceptor + H* + O2 | - |
Methylococcus capsulatus | methanol + acceptor + H2O | - |
? | |
methane + reduced acceptor + H* + O2 | - |
Methylococcus capsulatus Bath | methanol + acceptor + H2O | - |
? | |
propene + reduced acceptor + H+ + O2 | enzyme form sMMO | Methylococcus capsulatus | 1,2-epoxypropane + acceptor + H2O | - |
? | |
propene + reduced acceptor + H+ + O2 | enzyme form sMMO | Methylococcus capsulatus Bath | 1,2-epoxypropane + acceptor + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | pMMO subunit A has acetylene binding ability | Methylococcus capsulatus |
trimer | 1 * 47000 + 1 * 27000 + 1 * 25000, pMMO, mass spectrometry and SDS-PAGE | Methylococcus capsulatus |
Synonyms | Comment | Organism |
---|---|---|
MMO | - |
Methylococcus capsulatus |
pMMO | particulate, membrane-bound enzyme form | Methylococcus capsulatus |
sMMO | soluble, cytoplasmic enzyme form | Methylococcus capsulatus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Methylococcus capsulatus |