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Literature summary for 1.14.18.1 extracted from

  • Ni Eidhin, D.; Degn, P.; Obeirne, D.
    Characterization of polyphenol oxidase from rooster potato (Solanum tuberosum cv Rooster) (2010), J. Food Biochem., 34, 13-30.
    View publication on PubMed
Search for more literature for 1.14.18.1

Activating Compound

Activating Compound Comment Organism Structure
SDS 172% activity at 10 mM Solanum tuberosum

Inhibitors

Inhibitors Comment Organism Structure
ascorbic acid effective inhibitor, 99% inhibition at 1 mM Solanum tuberosum
benzoic acid 9% residual activity at 10 mM Solanum tuberosum
borax 71% residual activity at 10 mM Solanum tuberosum
CaCl2 69% residual activity at 10 mM Solanum tuberosum
Citric acid 10% residual activity at 10 mM Solanum tuberosum
EDTA 57% residual activity at 10 mM Solanum tuberosum
L-cysteine complete inhibition at 1 mM Solanum tuberosum
N-acetyl-L-cysteine 1% residual activity at 1 mM Solanum tuberosum
NaCl 84% residual activity at 10 mM Solanum tuberosum
potassium sorbate 78% residual activity at 10 mM Solanum tuberosum
Sodium metabisulfite complete inhibition at 1 mM; effective inhibitor, complete inhibition at 1 mM Solanum tuberosum
succinic acid 35% residual activity at 10 mM Solanum tuberosum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Solanum tuberosum the partially purified enzyme has both cresolase and catecholase activity. Activity is lower toward monophenols than diphenols ?
-
 ?

Organism

Organism UniProt Comment Textmining
Solanum tuberosum
-
 cultivar Rooster, rooster potato
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Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, phenyl Sepharose column chromatography, ion exchange chromatography, and hydroxyapatite column chromatography Solanum tuberosum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 the specific activity of the crude enzyme is 264 units/mg protein, the specific activity of the 64fold purified enzyme is16853 units/mg protein using 50 mM catechol as substrate in 50 mM sodium phosphate buffer, pH 7.0, at 25°C, one unit of PPO activity is defined as the change in absorbance of 0.001 per minute per ml of enzyme Solanum tuberosum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(-)-epicatechin + O2 119% activity at 2.5 mM substrate concentration Solanum tuberosum ?
-
 ?
2 catechol + O2 100% activity at 10 mM substrate concentration Solanum tuberosum 2 1,2-benzoquinone + 2 H2O
-
 ?
4-methylcatechol + O2 highest activity, 183% activity at 10 mM substrate concentration Solanum tuberosum 4-methyl-1,2-benzoquinone + H2O
-
 ?
caffeic acid + O2 31% activity at 2.5 mM substrate concentration Solanum tuberosum caffeoyl quinone + H2O
-
 ?
DL-DOPA + O2 39% activity at 2.5 mM substrate concentration Solanum tuberosum dopaquinone + H2O
-
 ?
L-tyrosine + O2 very low activity, 6% activity at 2.5 mM substrate concentration Solanum tuberosum L-DOPA + H2O
-
 ?
additional information the partially purified enzyme has both cresolase and catecholase activity. Activity is lower toward monophenols than diphenols Solanum tuberosum ?
-
 ?
additional information no activity with ferulic acid and phenol Solanum tuberosum ?
-
 ?
p-cresol + O2 20% activity at 2.5 mM substrate concentration Solanum tuberosum ?
-
 ?
pyrogallol + O2 92% activity at 10 mM substrate concentration Solanum tuberosum ?
-
 ?

Synonyms

Synonyms Comment Organism
polyphenol oxidase
-
 Solanum tuberosum
PPO
-
 Solanum tuberosum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Solanum tuberosum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
20 80 residual activity is higher than 50% after incubation at 20°C for 72 h, at 30°C for 48 h, at 40°C for 24 h, at 50°C for 2 h, and at 60°C for 15 min. Heating at 80°C for 15 min inactivates the enzyme Solanum tuberosum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 6.5
-
 Solanum tuberosum

pH Range

pH Minimum pH Maximum Comment Organism
4.5 7.5
-
 Solanum tuberosum

pH Stability

pH Stability pH Stability Maximum Comment Organism
2.5 3.5 the enzyme is most unstable at pH 2.5-3.5 retaining less than 10% of original activity after 2 h Solanum tuberosum
6 7.5 more than 50% activity is retained during storage for 72 h at pH 6.0-7.5 Solanum tuberosum