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Literature summary for 1.14.18.1 extracted from

  • Chen, C.Q.; Li, Z.C.; Pan, Z.Z.; Zhu, Y.J.; Yan, R.R.; Wang, Q.; Yan, J.H.; Chen, Q.X.
    Inactivation kinetics of polyphenol oxidase from pupae of blowfly (Sarcophaga bullata) in the dimethyl sulfoxide solution (2009), Appl. Biochem. Biotechnol., 160, 2166-2174.
    View publication on PubMed
Search for more literature for 1.14.18.1

Inhibitors

Inhibitors Comment Organism Structure
DMSO low concentrations of DMSO (lower than 3.5 M) lead to reversible mixed-type inhibition of the enzyme, 68.0% residual activity at 1.4 M, 52.6% residual activity at 2.1 M, 36.6% residual activity at 2.8 M, complete inhibition at 5.6 M Sarcophaga bullata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.838
-
 L-Dopa at 30°C Sarcophaga bullata

Organism

Organism UniProt Comment Textmining
Sarcophaga bullata
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate fractionation, diethylaminoethyl cellulose column chromatography, and Sephadex G-100 gel filtration Sarcophaga bullata

Source Tissue

Source Tissue Comment Organism Textmining
pupa
-
 Sarcophaga bullata
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
772
-
 at 30°C Sarcophaga bullata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-DOPA + O2
-
 Sarcophaga bullata dopaquinone + H2O
-
 r
L-tyrosine + O2
-
 Sarcophaga bullata dopaquinone + H2O
-
 ?

Synonyms

Synonyms Comment Organism
polyphenol oxidase
-
 Sarcophaga bullata
PPO
-
 Sarcophaga bullata

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2350
-
 at 30°C Sarcophaga bullata DMSO