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Literature summary for 1.14.18.1 extracted from

  • Tembe, S.; Kubal, B.S.; Karve, M.; DSouza, S.F.
    Glutaraldehyde activated eggshell membrane for immobilization of tyrosinase from Amorphophallus companulatus: application in construction of electrochemical biosensor for dopamine (2008), Anal. Chim. Acta, 612, 212-217.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
dopamine soluble enzyme Amorphophallus paeoniifolius var. campanulatus
0.67
-
dopamine using tyrosinase immobilized on eggshell membrane. Km is 0.67 mM and Vmax 0.08 mM min-1. Increase in Km (compared to soluble enzyme for which Km is 0.20 mM) indicate that the affinity of enzyme for substrate has decreased to some extent after immobilization, which may be due to glutaraldehyde treatment Amorphophallus paeoniifolius var. campanulatus

Localization

Localization Comment Organism GeneOntology No. Textmining

Organism

Organism UniProt Comment Textmining
Amorphophallus paeoniifolius var. campanulatus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining

Storage Stability

Storage Stability Organism
Shelf life of immobilized tyrosinase system is found to be more than 6 months when stored in phosphate buffer at 4°C. Immobilization on preactivated eggshell membrane followed by enzyme adsorption is a simple and a fast procedure to prepare stable immobilized tyrosinase. Amorphophallus paeoniifolius var. campanulatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
dopamine + 1/2 O2
-
Amorphophallus paeoniifolius var. campanulatus dopamine quinone + H2O
-
?

Synonyms

Synonyms Comment Organism
polyphenol oxidase a binuclear copper containing metalloprotein Amorphophallus paeoniifolius var. campanulatus
tyrosinase
-
Amorphophallus paeoniifolius var. campanulatus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 45 membrane bound enzyme exhibits consistent activity in the temperature range 20-45°C Amorphophallus paeoniifolius var. campanulatus

pH Range

pH Minimum pH Maximum Comment Organism
5 6.5 studies on effect of pH show retention of more than 90% activity over a pH range 5.0-6.5 Amorphophallus paeoniifolius var. campanulatus