Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | 200-400 mM, activates | Bacillus thuringiensis | |
SDS | activates | Bacillus thuringiensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alpha,alpha'-dipyridyl | 5 mM, 44% inhibition | Bacillus thuringiensis | |
diethyldithiocarbamate | 0.001 mM, 10% inhibition | Bacillus thuringiensis | |
EDTA | below 2 mM, 66% inhibition at 0.5 mM, 28% inhibition at 1 mM, no inhibition at 2 mM | Bacillus thuringiensis | |
p-phenanthroline | 5 M, complete inhibition | Bacillus thuringiensis | |
Phenylthiourea | 0.001 mM, 26% inhibition | Bacillus thuringiensis | |
Sodium fluoride | 5 mM, complete inhibition | Bacillus thuringiensis | |
Triton X-100 | 0.5% 87% inhibition | Bacillus thuringiensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.563 | - |
L-Tyr | pH 8.5, 42°C | Bacillus thuringiensis | |
0.768 | - |
L-Dopa | pH 8.5, 42°C | Bacillus thuringiensis | |
0.892 | - |
dopamine | pH 8.5, 42°C | Bacillus thuringiensis | |
34.05 | - |
catechol | pH 8.5, 42°C | Bacillus thuringiensis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 10 mM, increases activity. Copper-containing enzyme | Bacillus thuringiensis | |
Mg2+ | 10 mM, increases activity | Bacillus thuringiensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
14000 | - |
x * 14000, SDS-PAGE | Bacillus thuringiensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus thuringiensis | - |
subsp. Kurstaki | - |
Purification (Comment) | Organism |
---|---|
- |
Bacillus thuringiensis |
Storage Stability | Organism |
---|---|
4°C, 40% loss of activity after 30 days | Bacillus thuringiensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4-dihydroxymandelic acid + O2 | 9% of the activity with L-dopa | Bacillus thuringiensis | ? | - |
? | |
3,4-dihydroxyphenylacetic acid + O2 | 12% of the activity with L-dopa | Bacillus thuringiensis | ? | - |
? | |
4-methyl catechol + O2 | 5% of the activity with L-dopa | Bacillus thuringiensis | 4-methyl-o-benzoquinone + H2O | - |
? | |
catechol + 1/2 O2 | 24% of the activity with L-dopa | Bacillus thuringiensis | 1,2-benzoquinone + H2O | - |
? | |
dopamine + 1/2 O2 | 96% of the activity with L-dopa | Bacillus thuringiensis | dopamine quinone + H2O | - |
? | |
L-dopa + 1/2 O2 | - |
Bacillus thuringiensis | L-dopaquinone + H2O | - |
? | |
L-Tyr + O2 + AH2 | 10% of the activity with L-dopa | Bacillus thuringiensis | L-dopa + H2O + A | - |
? | |
resorcinol + O2 | 3% of the activity with L-dopa | Bacillus thuringiensis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 14000, SDS-PAGE | Bacillus thuringiensis |
Synonyms | Comment | Organism |
---|---|---|
tyrosinase | - |
Bacillus thuringiensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
- |
Bacillus thuringiensis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | 85 | 55°C: about 75% of maximal activity, 85°C: about 80% of maximal activity | Bacillus thuringiensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
10 min, 25% loss of activity | Bacillus thuringiensis |
100 | - |
10 min, 30% loss of activity. Complete loss of activity after 15 min | Bacillus thuringiensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Bacillus thuringiensis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 9.5 | pH 8.0: about 30% of maximal activity, pH 9.0: maximal activity, pH 9.5: no activity | Bacillus thuringiensis |