Literature summary for 1.14.18.1 extracted from

Ito, M.; Oda, K.
An organic solvent resistant tyrosinase from Streptomyces sp. REN-21: purification and characterization (2000), Biosci. Biotechnol. Biochem., 64, 261-267.

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Inhibitors

Inhibitors	Comment	Organism	Structure
5-hydroxy-2-(hydroxymethyl)-2H-pyran-4-one	-	Streptomyces sp.
arbutin	no inhibition by 1 mM arbutin	Streptomyces sp.
azide	no inhibition by sodium azide	Streptomyces sp.
diethyldithiocarbamate	-	Streptomyces sp.
DL-dithiothreitol	-	Streptomyces sp.
EDTA	no inhibition EDTA	Streptomyces sp.
ethanol	50% ethanol, 56% inhibition	Streptomyces sp.
L-cysteine	-	Streptomyces sp.
methanol	50% inhibition in 50% methanol	Streptomyces sp.
additional information	not inhibited by 1 mM arbutin, or 0.2 mM Cu2+, Fe3+, Zn2+, Mg2+ and Ca2+	Streptomyces sp.

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.25	-	L-tyrosine	-	Streptomyces sp.
1.64	-	DL-tyrosine	-	Streptomyces sp.
1.98	-	D-tyrosine	-	Streptomyces sp.
4.14	-	L-Dopa	-	Streptomyces sp.
9.56	-	DL-dopa	-	Streptomyces sp.
28.7	-	D-Dopa	-	Streptomyces sp.

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
32000	-	x * 32000, SDS-PAGE	Streptomyces sp.

Organic Solvent Stability

Organic Solvent	Comment	Organism
Acetone	no loss of activity after 20 h at 30°C in 20% acetone	Streptomyces sp.
dimethyl sulfoxide	no loss of activity after 20 h at 30°C in 20% dimethylsulfoxide	Streptomyces sp.
Ethanol	30% ethanol, 30°C, pH 7.0, 17% loss of activity after 20 h	Streptomyces sp.

Organism

Organism	UniProt	Comment	Textmining
Mushroom	-	-	-
Streptomyces sp.	-	REN-21, organic solvent resistant enzyme	-

Purification (Commentary)

Purification (Comment)	Organism
ammonium sulfate, DEAE-cellulose, QAE-Sephadex A-50, Toyopearl HW-55	Streptomyces sp.

Source Tissue

Source Tissue	Comment	Organism	Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
130	-	-	Streptomyces sp.

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
D-dopa + 1/2 O2	-	Streptomyces sp.	dopaquinone + H2O	-	r
D-dopa + O2	-	Streptomyces sp.	dopaquinone + H2O	-	r
D-tyrosine + O2	-	Streptomyces sp.	dopaquinone + H2O	-	r
D-tyrosine + O2	-	Streptomyces sp.	?	-	r
DL-dopa + 1/2 O2	-	Streptomyces sp.	dopaquinone + H2O	-	r
DL-DOPA + O2	-	Streptomyces sp.	dopaquinone + H2O	-	r
DL-tyrosine + O2	-	Streptomyces sp.	dopaquinone + H2O	-	r
L-epicatechin + O2	155% of activity with L-dopa	Streptomyces sp.	?	-	?
L-tyrosine + O2 + AH2	-	Streptomyces sp.	L-dopa + H2O + A	-	?

Subunits

Subunits	Comment	Organism
?	-	Mushroom
?	x * 32000, SDS-PAGE	Streptomyces sp.

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
35	-	with L-dopa	Streptomyces sp.
40	-	-	Mushroom

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
2.27	-	D-tyrosine	-	Streptomyces sp.
74.2	-	DL-tyrosine	-	Streptomyces sp.
81.4	-	L-tyrosine	-	Streptomyces sp.
117	-	D-Dopa	-	Streptomyces sp.
1120	-	L-Dopa	-	Streptomyces sp.
1170	-	DL-dopa	-	Streptomyces sp.

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	with L-dopa	Streptomyces sp.

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Release 2023.1 (January 2023)