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Literature summary for 1.14.18.1 extracted from

  • Sharma, R.C.; Ali, R.
    Isolation and characterization of catechol oxidase from Solanum melongena (1980), Phytochemistry, 19, 1597-1600.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
2,3-dimercapto-1-propanol 2 mM, 93% inhibition Solanum melongena
2-mercaptoethanol 2 mM, 93% inhibition Solanum melongena
L-ascorbic acid 2 mM, 78% inhibition Solanum melongena
L-cysteine 2 mM, 95% inhibition Solanum melongena

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.7
-
catechol
-
Solanum melongena
6.25
-
Dopa
-
Solanum melongena

Localization

Localization Comment Organism GeneOntology No. Textmining
particle-bound
-
Solanum melongena
-
-
soluble
-
Solanum melongena
-
-

Organism

Organism UniProt Comment Textmining
Solanum melongena
-
eggplant
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate, Sephadex G-200 Solanum melongena

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
Solanum melongena
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
336
-
-
Solanum melongena

Storage Stability

Storage Stability Organism
0°C, presence of SDS protects against decomposition Solanum melongena

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
catechol + 1/2 O2
-
Solanum melongena 1,2-benzoquinone + H2O
-
?
dopa + 1/2 O2
-
Solanum melongena dopaquinone + H2O
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
substrate catechol, 2 pH-maxima at 6.5 and 7.5 Solanum melongena
6.5
-
substrate dopa, 2 pH-maxima at pH 6.5 and pH 7.9 Solanum melongena
7.5
-
substrate catechol, 2 pH-maxima at 6.5 and 7.5 Solanum melongena
7.9
-
substrate dopa, 2 pH-maxima at pH 6.5 and pH 7.9 Solanum melongena

pH Range

pH Minimum pH Maximum Comment Organism
5 9 pH 5.0: about 60% of maximal activity, pH 9.0: about 40% of maximal activity, dopa as substrate Solanum melongena
5 8 pH 5.0: about 55% of maximal activity, pH 9.0: about 15% of maximal activity, catechol as substrate Solanum melongena