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Literature summary for 1.14.18.1 extracted from

  • Birse, C.E.; Clutterbuck, A.J.
    N-acetyl-6-hydroxytryptophan oxidase, a developmentally controlled phenol oxidase from Aspergillus nidulans (1990), J. Gen. Microbiol., 136, 1725-1730.
    View publication on PubMed
Search for more literature for 1.14.18.1

Inhibitors

Inhibitors Comment Organism Structure
1-Phenyl-2-thiourea 1 mM, 94% inhibition Aspergillus nidulans
8-hydroxyquinoline 3 mM, 44% inhibition Aspergillus nidulans
azide no inhibition by sodium azide Aspergillus nidulans
diethyldithiocarbamate 0.1 mM, complete inhibition Aspergillus nidulans
Metabisulfite 0.1 mM, 99% inhibition Aspergillus nidulans
o-phenanthroline hydrate 3 mM, 30% inhibition Aspergillus nidulans
phenylhydrazine 1 mM, 86% inhibition, noncompetitive inhibition Aspergillus nidulans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2 14 Hydroquinone monomethylether range of estimated values for crude or purified extracts Aspergillus nidulans

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Aspergillus nidulans
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
copper 2.1 copper atoms per polypeptide, enzyme form B Aspergillus nidulans
Zn2+ 0.9 mol per polypeptide Aspergillus nidulans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
 x * 48000, enzyme form B, SDS-PAGE Aspergillus nidulans
50000
-
 x * 50000, enzyme form A, SDS-PAGE Aspergillus nidulans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N-acetyl-6-hydroxytryptophan + O2 Aspergillus nidulans
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus nidulans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE-Sepharose, Phenyl Sepharose, enzyme form A and B Aspergillus nidulans

Source Tissue

Source Tissue Comment Organism Textmining
conidiophore
-
 Aspergillus nidulans
-
culture filtrate
-
 Aspergillus nidulans
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 enzyme form A, 480.0 DELTA absorbance 470 nm/min/mg, enzyme form B, 258.6 DELTA absorbance 470 nm/min/mg Aspergillus nidulans

Storage Stability

Storage Stability Organism
4°C, 3 weeks, no loss of activity Aspergillus nidulans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylcatechol + O2
-
 Aspergillus nidulans 4-methyl-o-benzoquinone + H2O
-
 ?
catechol + 1/2 O2
-
 Aspergillus nidulans 1,2-benzoquinone + H2O
-
 ?
chlorogenic acid + O2
-
 Aspergillus nidulans chlorogenoquinone + H2O
-
 ?
D-catechin + O2
-
 Aspergillus nidulans ?
-
 ?
hydroquinone monomethylether + O2
-
 Aspergillus nidulans quinone monomethylether
-
 ?
N-acetyl-6-hydroxytryptophan + O2
-
 Aspergillus nidulans ?
-
 ?
o-methoxyphenol + O2 + AH2 trivial name guaiacol Aspergillus nidulans 1,2-dihydroxy-3-methoxybenzene + H2O + A
-
 ?

Subunits

Subunits Comment Organism
? x * 48000, enzyme form B, SDS-PAGE Aspergillus nidulans
? x * 50000, enzyme form A, SDS-PAGE Aspergillus nidulans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 with hydroquinone monomethylether Aspergillus nidulans