Any feedback?
Literature summary for 1.14.17.4 extracted from
- Identification and active site analysis of the 1-aminocyclopropane-1-carboxylic acid oxidase catalysing the synthesis of ethylene in Agaricus bisporus (2014), Biochim. Biophys. Acta, 1840, 120-128.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| bicarbonate | the enzyme is completely dependent on bicarbonate | Agaricus bisporus |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Agaricus bisporus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| G289S | the enzyme activity of the mutant is 3times as high as that of the wild type enzyme | Agaricus bisporus |
| H216D | the mutant shows 76% of wild type activity | Agaricus bisporus |
| H216D/D218E | the mutant shows 78% of wild type activity | Agaricus bisporus |
| H216D/D218E/H273Q | the mutant does not completely lose catalytic activity | Agaricus bisporus |
| H273Q | the mutant shows 62% of wild type activity | Agaricus bisporus |
| R287G | the activity of the mutant is remarkably decreased to nearly 40% compared to the wild type enzyme | Agaricus bisporus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.25 | - |
1-aminocyclopropane-1-carboxylate | at pH and °C | Agaricus bisporus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | in the absence of Fe2+, the activity is 2.4fold lower than that in the steady-state reaction when 0.0025 mM Fe2+ is added (maximum activity at 0.01 mM Fe2+) | Agaricus bisporus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-aminocyclopropane-1-carboxylate + ascorbate + O2 | Agaricus bisporus | - |
ethylene + cyanide + dehydroascorbate + CO2 + 2 H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Agaricus bisporus | H9ZYN5 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| fruit body | - |
Agaricus bisporus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-aminocyclopropane-1-carboxylate + ascorbate + O2 | - |
Agaricus bisporus | ethylene + cyanide + dehydroascorbate + CO2 + 2 H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 1-aminocyclopropane-1-carboxylic acid oxidase | - |
Agaricus bisporus |
| ACC oxidase | - |
Agaricus bisporus |
| ACO | - |
Agaricus bisporus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme does not require ascorbate | Agaricus bisporus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
