Cloned (Comment) | Organism |
---|---|
into the vector pGEM-T Easy and subsequently into pProEX-1 for expression of the protein in Escherichia coli BL21 cells | Malus domestica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08939 | - |
1-aminocyclopropane-1-carboxylic acid | - |
Malus domestica | |
0.2445 | - |
1-aminocyclopropane-1-carboxylic acid | - |
Malus domestica | |
0.401 | - |
1-aminocyclopropane-1-carboxylic acid | - |
Malus domestica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | - |
Malus domestica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35350 | - |
determined by SDA-PAGE and Western blot analysis | Malus domestica |
36310 | - |
determined by SDA-PAGE and Western blot analysis | Malus domestica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-aminocyclopropane-1-carboxylic acid + ascorbate + O2 | Malus domestica | - |
ethylene + cyanide + dehydroascorbate + CO2 + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Malus domestica | - |
- |
- |
Malus domestica | O48882 | - |
- |
Malus domestica | Q00985 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant ACO is purified by affinity chromatography using nickel nitrilotriacetic acid resin, furthermore Sephadex G-25 resin and a Mono-Q column are used, in addition ACO is extracted from apple leaf and fruit tissue | Malus domestica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | - |
Malus domestica | - |
leaf | - |
Malus domestica | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-aminocyclopropane-1-carboxylic acid + ascorbate + O2 | - |
Malus domestica | ethylene + cyanide + dehydroascorbate + CO2 + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
1-aminocyclopropane-1-carboxylic acid oxidase | - |
Malus domestica |
ACC oxidase | - |
Malus domestica |
ACO | - |
Malus domestica |
MD-ACO1 | - |
Malus domestica |
MD-ACO2 | - |
Malus domestica |
MD-ACO3 | - |
Malus domestica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0344 | - |
1-aminocyclopropane-1-carboxylic acid | - |
Malus domestica | |
0.066 | - |
1-aminocyclopropane-1-carboxylic acid | - |
Malus domestica | |
0.0914 | - |
1-aminocyclopropane-1-carboxylic acid | - |
Malus domestica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
- |
Malus domestica |
7.5 | - |
- |
Malus domestica |
7.8 | - |
- |
Malus domestica |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.5 | - |
Malus domestica |
6.5 | 8 | - |
Malus domestica |