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Literature summary for 1.14.17.4 extracted from

  • Vioque, B.; Castellano, J.M.
    Extraction and biochemical characterization of 1-aminocyclopropane-1-carboxylic acid oxidase from pear (1994), Physiol. Plant., 90, 334-338.
No PubMed abstract available
Search for more literature for 1.14.17.4

Activating Compound

Activating Compound Comment Organism Structure
ascorbate absolutely dependent on Pyrus communis
CO2 absolutely dependent on Pyrus communis
CO2 best at 14% or higher Pyrus communis
O2 dependent on Pyrus communis
Triton X-100 best at 0.8% or above Pyrus communis

Inhibitors

Inhibitors Comment Organism Structure
alpha-aminoisobutyric acid
-
 Pyrus communis
Co2+
-
 Pyrus communis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.057
-
 1-aminocyclopropane-1-carboxylate pH 6.7, 30°C Pyrus communis

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ absolutely required Pyrus communis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-aminocyclopropane-1-carboxylate + ascorbate + O2 Pyrus communis
-
 ethylene + cyanide + dehydroascorbate + CO2 + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Pyrus communis
-
 cv.Blanquilla
-

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
 Pyrus communis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0047
-
 crude enzyme extract Pyrus communis
0.006
-
 pear fruit prior homogenization Pyrus communis

Storage Stability

Storage Stability Organism
-20°C, 30% glycerol, stable for 11 days Pyrus communis
2°C, 30% glycerol, loss of 30% activity after 24 h Pyrus communis
2°C, 30% glycerol, loss of 85% activity after 7 h Pyrus communis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-aminocyclopropane-1-carboxylate + ascorbate + O2
-
 Pyrus communis ethylene + cyanide + dehydroascorbate + CO2 + H2O
-
 ?
1-aminocyclopropane-1-carboxylate + L-ascorbate + O2 absolutely dependent on CO2 Pyrus communis ethylene + cyanide + dehydroascorbate + CO2 + H2O
-
 ?
1-aminocyclopropane-1-carboxylate + L-ascorbate + O2 i.e. ACC Pyrus communis ethylene + cyanide + dehydroascorbate + CO2 + H2O
-
 ?
1-aminocyclopropane-1-carboxylate + L-ascorbate + O2 absolutely dependent on ascorbate Pyrus communis ethylene + cyanide + dehydroascorbate + CO2 + H2O
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
 2 optima at 28°C and 38°C Pyrus communis
38
-
 2 optima at 28°C and 38°C Pyrus communis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.2
-
 alpha-aminoisobutyric acid pH 6.7, 30°C Pyrus communis