Cloned (Comment) | Organism |
---|---|
expression in CHO DG44 cells | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
H172A | mutation in the copper center of domain one, no activity in presence of H2O2 | Rattus norvegicus |
H242A | mutation in the copper center of domain two, no activity in presence of H2O2 | Rattus norvegicus |
additional information | only the residues 42-356 carrying the monoogenase domain was expressed | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0051 | - |
dansyl-D-Tyr-Val-Gly | 100 mM MES, pH 5.5, 0.2 mM dansyl-D-Tyr-Val-Gly, 5 microM CuSO4, ascorbate | Rattus norvegicus | |
0.8 | - |
dansyl-D-Tyr-Val-Gly | 100 mM MES, pH 5.5, 0.2 mM dansyl-D-Tyr-Val-Gly, 5 microM CuSO4, 1 mM H2O2 | Rattus norvegicus | |
2.78 | - |
H2O2 | 100 mM MES, pH 5.5, 0.2 mM dansyl-D-Tyr-Val-Gly, 5 microM CuSO4, H2O2 | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | requirred | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
two chromatoography steps | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dansyl-D-Tyr-Val-Gly + ascorbate + O2 | - |
Rattus norvegicus | dansyl-D-Tyr-Val-2-hydroxyglycine + dehydroascorbate + H2O | - |
? | |
dansyl-D-Tyr-Val-Gly + H2O2 | peptidylglycine monooxygenase is able to catalyze the hydroxylation of peptidylglycine substrates starting from the oxidized enzyme and using hydrogen peroxide as the only source of oxygen | Rattus norvegicus | dansyl-D-Tyr-Val-2-hydroxyglycine + H2O | - |
? | |
N-dansyl-L-Tyr-L-Val-Gly + ascorbate + O2 | - |
Rattus norvegicus | N-dansyl-L-Tyr-L-Val-2-hydroxyglycine + dehydroascorbate + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
peptidylglycine alpha-hydroxylating monooxygenase | - |
Rattus norvegicus |
peptidylglycine monooxygenase | - |
Rattus norvegicus |
PHMcc | - |
Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5 | - |
(25R)-3beta-hydroxycholest-5-en-27-oate | 100 mM MES, pH 5.5, 0.2 mM dansyl-D-Tyr-Val-Gly, 5 microM CuSO4, H2O2 | Rattus norvegicus | |
9.2 | - |
N-dansyl-L-Tyr-L-Val-Gly | 100 mM MES, pH 5.5, 0.2 mM dansyl-D-Tyr-Val-Gly, 5 microM CuSO4, ascorbate | Rattus norvegicus |