Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diethyldithiocarbamate | copper chelator | Bos taurus | |
additional information | activity is not affected by pepstatin A, phenylmethylsulfonyl fluoride, and soybean trypsin inhibitor | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.022 | - |
dabsyl-Gly-Phe-Gly | form 2 | Bos taurus | |
0.143 | - |
dabsyl-Gly-Phe-Gly | form 1 | Bos taurus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | optimal activity at 0.016 mM | Bos taurus | |
Cu2+ | Cu2+ is absolutely required for optimal activity | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
49000 | - |
form 1, gel filtration | Bos taurus |
69000 | - |
form 2, gel filtration | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
peptidylglycine + ascorbate + O2 | Bos taurus | - |
peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
hypothalamus | 2 forms, distinguishable by molecular weight | Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-dimethylaminoazobenzene-4'-sulfonyl-Gly-L-Phe-Gly + ascorbate + O2 | - |
Bos taurus | 4-dimethylaminoazobenzene-4'-sulfonyl-Gly-L-Phe-2-hydroxyglycine + dehydroascorbate + H2O | - |
? | |
peptidylglycine + ascorbate + O2 | - |
Bos taurus | peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O | - |
? | |
peptidylglycine + ascorbate + O2 | COOH-terminal glycine | Bos taurus | peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
2 optima: at pH 6.0 and at pH 7.5 | Bos taurus |
7.5 | - |
2 optima: at pH 6.0 and at pH 7.5 | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ascorbate | dependent on | Bos taurus |