Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dithiothreitol | - |
Xenopus laevis | |
EDTA | - |
Xenopus laevis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00035 | - |
acetyl-L-Tyr-L-Phe-Gly | in presence of 0.25 mM ascorbate | Xenopus laevis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | a copper protein | Xenopus laevis | |
Cu2+ | Cu2+ is absolutely required for optimal activity | Xenopus laevis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
SDS-PAGE, gel filtration | Xenopus laevis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
peptidylglycine + ascorbate + O2 | Xenopus laevis | - |
peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xenopus laevis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Xenopus laevis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
skin | - |
Xenopus laevis | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Xenopus laevis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-L-Tyr-L-Phe-Gly + ascorbate + O2 | - |
Xenopus laevis | acetyl-L-Tyr-L-Phe-2-hydroxyglycine + dehydroascorbate + H2O | - |
r | |
peptidylglycine + ascorbate + O2 | - |
Xenopus laevis | peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O | - |
? | |
peptidylglycine + ascorbate + O2 | diverse synthetic substrates with C-terminal glycine | Xenopus laevis | peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O | - |
? | |
peptidylglycine + ascorbate + O2 | COOH-terminal glycine | Xenopus laevis | peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 39000, SDS-PAGE | Xenopus laevis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Xenopus laevis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | - |
Xenopus laevis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ascorbate | dependent on | Xenopus laevis |