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Literature summary for 1.14.17.3 extracted from

  • Murthy, A.S.N.; Mains, R.E.; Eipper, B.A.
    Purification and characterization of peptidylglycine alpha-amidating monooxygenase from bovine neurointermediate pituitary (1986), J. Biol. Chem., 261, 1815-1822.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
diethyldithiocarbamate
-
Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.007
-
D-Tyr-Val-Gly PAM-B, with 1.25 mM ascorbate Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
secretory granule
-
Bos taurus 30141
-

Metals/Ions

Metals/Ions Comment Organism Structure
Cu2+ a copper protein Bos taurus
Cu2+ Cu2+ is absolutely required for optimal activity Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
PAM-B, heterogeneous in size, gel filtration Bos taurus
48000
-
1 * 48000, PAM-A, heterogeneous in size, SDS-PAGE Bos taurus
54000
-
PAM-A, heterogeneous in size, gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
peptidylglycine + ascorbate + O2 Bos taurus
-
peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
2 forms: A and B
-

Purification (Commentary)

Purification (Comment) Organism
forms A and B differing in MW and charge Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
pituitary gland
-
Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.038
-
purified PAM-A Bos taurus
0.115
-
purified PAM-B Bos taurus
1.15
-
-
Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-Tyr-Val-Gly + ascorbate + O2
-
Bos taurus D-Tyr-Val-2-hydroxyglycine + dehydroascorbate + H2O
-
?
peptidylglycine + ascorbate + O2
-
Bos taurus peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
?
peptidylglycine + ascorbate + O2 COOH-terminal glycine Bos taurus peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O
-
?

Subunits

Subunits Comment Organism
monomer 1 * 38000, PAM-B, heterogeneous in size, SDS-PAGE Bos taurus
monomer 1 * 48000, PAM-A, heterogeneous in size, SDS-PAGE Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
PAM-A Bos taurus
9 9.5 PAM-B Bos taurus

pH Range

pH Minimum pH Maximum Comment Organism
7.5 10 pH 7.5: about 50% of activity maximum, pH 10: about 60% of activity maximum, PAM-B Bos taurus

Cofactor

Cofactor Comment Organism Structure
ascorbate dependent on Bos taurus