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Literature summary for 1.14.17.3 extracted from
- Peptidyl-glycine alpha-amidating mono-oxygenase activity towards a gonadotropin-releasing-hormone C-terminal peptide substrate, in subcellular fractions of sheep brain and pituitary (1988), Biochem. J., 251, 251-259.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-Tyr-Pro-Gly-Gly | inhibition of activity with D-Tyr-Val-Gly | Ovis aries |  |
| D-Tyr-Val-Gly | inhibition of activity with D-Tyr-Pro-Gly | Ovis aries | |
| EDTA | - |
Ovis aries | |
| NaN3 | - |
Ovis aries | |
| p-hydroxymercuribenzoate | slightly | Ovis aries | |
| phosphate | - |
Ovis aries | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.033 | - |
D-Tyr-Pro-Gly-Gly | assayed without ascorbate and catalase, brain enzyme | Ovis aries | |
| 0.068 | - |
D-Tyr-Pro-Gly-Gly | assayed without ascorbate and catalase, pituitary enzyme | Ovis aries | |
| 0.075 | - |
D-Tyr-Pro-Gly-Gly | assayed with ascorbate and 0.1mg/ml catalase, pituitary enzyme | Ovis aries | |
| 0.289 | - |
D-Tyr-Pro-Gly-Gly | assayed with ascorbate and 0.1 mg/ml catalase, brain enzyme | Ovis aries | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| secretory granule | synaptosomal and neurosecretory granules | Ovis aries | 30141 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | Cu2+ strongly stimulates activity | Ovis aries | |
| Cu2+ | a copper protein | Ovis aries | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| peptidylglycine + ascorbate + O2 | Ovis aries | - |
peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ovis aries | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| hypothalamus | - |
Ovis aries | - |
| pituitary gland | - |
Ovis aries | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
very low activity in crude subcellular preparations | Ovis aries |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-Tyr-Pro-Gly-Gly + ascorbate + O2 | synthetic peptide | Ovis aries | D-Tyr-Pro-Gly-2-hydroxyglycine + dehydroascorbate + H2O | - |
? | |
| D-Tyr-Pro-Gly-Gly + ascorbate + O2 | optimal activity in sulfonic acid buffers | Ovis aries | D-Tyr-Pro-Gly-2-hydroxyglycine + dehydroascorbate + H2O | - |
? | |
| D-Tyr-Val-Gly + ascorbate + O2 | - |
Ovis aries | D-Tyr-Val-2-hydroxyglycine + dehydroascorbate + H2O | - |
? | |
| peptidylglycine + ascorbate + O2 | - |
Ovis aries | peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O | - |
? | |
| peptidylglycine + ascorbate + O2 | COOH-terminal glycine | Ovis aries | peptidyl(2-hydroxyglycine) + dehydroascorbate + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| peptidylglycine alpha-amidating mono-oxygenase | - |
Ovis aries |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | dependent on Cu2+-concentration | Ovis aries |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ascorbate | dependent on | Ovis aries | |
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