Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | pronase is most effective for activation. Trypsin, chymotrypsin, V8 protease and lysylendoprotease also activate PTMO, but the activity obtained using these proteases is only 5% of that with pronase | Ralstonia solanacearum |
Cloned (Comment) | Organism |
---|---|
pBR322 containing the open reading frame of PTMO, PCR products inserted into the EcoRV site of pBluescript vector, expressed from vector pET 22b(+) in Escherichia coli BL21(DE3) | Ralstonia solanacearum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
162000 | - |
gel filtration | Ralstonia solanacearum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ralstonia solanacearum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
on Ni column | Ralstonia solanacearum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-methionine + ? + O2 | relative high activity with pronase-treated PTMO | Ralstonia solanacearum | ? | - |
? | |
L-phenylalanine + tetrahydropteridine + O2 | purified PTMO shows no activity, relative high activity with pronase-treated PTMO | Ralstonia solanacearum | L-tyrosine + dihydropteridine + H2O | - |
? | |
L-tryptophan + tetrahydrobiopterin + O2 | relative high activity with pronase-treated PTMO | Ralstonia solanacearum | 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin | - |
? | |
L-tyrosine + tetrahydropteridine + O2 | relative high activity with pronase-treated PTMO | Ralstonia solanacearum | ? | - |
? | |
additional information | pronase-treated PTMO shows less than 5% activity of that of L-phenylalanine for L-lysine, L-histidine and L-threonine | Ralstonia solanacearum | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PTMO | - |
Ralstonia solanacearum |
putative tryptophan monooxygenase | - |
Ralstonia solanacearum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Ralstonia solanacearum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.5 | - |
Ralstonia solanacearum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
tetrahydrobiopterin | - |
Ralstonia solanacearum |