Literature summary for 1.14.16.4 extracted from

Kuhn, D.M.; Sakowski, S.A.; Geddes, T.J.; Wilkerson, C.; Haycock, J.W.
Phosphorylation and activation of tryptophan hydroxylase 2: identification of serine-19 as the substrate site for calcium, calmodulin-dependent protein kinase II (2007), J. Neurochem., 103, 1567-1573.

Search for more literature for 1.14.16.4

Activating Compound

Activating Compound	Comment	Organism	Structure
additional information	phosphorylation of TPH2 at Ser19 within the regulatory domain of the enzyme by calmodulin-dependent protein kinase II activates the enzyme	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
expression of GST-tagged TPH2 in Escherichia coli	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
S19A	site-directed mutagenesis, the mutant is not phpsphorylated by calmodulin-dependent protein kinase II and not activates, but basal activity and catalytic function is unaltered	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
L-tryptophan + tetrahydrobiopterin + O2	Homo sapiens	initial and rate-limiting enzyme in the biosynthesis of serotonin	5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	isozymes TPH1 and TPPH2	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
phosphoprotein	phosphorylation of TPH2 at Ser19 by calmodulin-dependent protein kinase II activates the enzyme	Homo sapiens

Purification (Commentary)

Purification (Comment)	Organism
recombinant GST-tagged TPH2 from Escherichia coli by glutathione affinity chromatography	Homo sapiens

Source Tissue

Source Tissue	Comment	Organism	Textmining
additional information	TPH1 is found in the periphery and pineal gland whereas TPH2 is expressed specifically in the central nervous system	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-tryptophan + 6-methyl-tetrahydropterin + O2	-	Homo sapiens	5-hydroxy-L-tryptophan + 6-methyl-4a-hydroxytetrahydropterin	-	?
L-tryptophan + tetrahydrobiopterin + O2	-	Homo sapiens	5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin	-	?
L-tryptophan + tetrahydrobiopterin + O2	initial and rate-limiting enzyme in the biosynthesis of serotonin	Homo sapiens	5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin	-	?

Synonyms

Synonyms	Comment	Organism
TPH2	-	Homo sapiens
tryptophan hydroxylase 2	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	assay at	Homo sapiens

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Release 2023.1 (January 2023)