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Literature summary for 1.14.16.4 extracted from

  • McKinney, J.; Knappskog, P.M.; Pereira, J.; Ekern, T.; Toska, K.; Kuitert, B.B.; Levine, D.; Gronenborn, A.M.; Martinez, A.; Haavik, J.
    Expression and purification of human tryptophan hydroxylase from Escherichia coli and Pichia pastoris (2004), Protein Expr. Purif., 33, 185-194.
    View publication on PubMed
Search for more literature for 1.14.16.4

Cloned(Commentary)

Cloned (Comment) Organism
expression in escherichia coli and Pichia pastoris, His-Patch-thioredoxin-TPH, 6His-TPH or as a fusion protein with maltose-binding protein Homo sapiens

General Stability

General Stability Organism
tryptophan and glycerol stabilize Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
(6R)-5,6,7,8-tetrahydrobiopterin
-
 Homo sapiens
dithiothreitol
-
 Homo sapiens
Fe2+
-
 Homo sapiens
L-tryptophan substrate inhibition above 0.1 mM Homo sapiens
additional information no substrate inhibition by (6R)-L-erytho-5,6,7,8-tetrahydrobiopterin Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0075
-
 L-tryptophan TPH as fusion protein with maltose-binding protein expressed in Escherichia coli Homo sapiens
0.0185
-
 L-tryptophan TPH expressed in Pichia pastoris Homo sapiens
0.027
-
 (6R)-L-erytho-5,6,7,8-tetrahydrobiopterin TPH as fusion protein with maltose-binding protein expressed in Escherichia coli Homo sapiens
0.039
-
 (6R)-L-erytho-5,6,7,8-tetrahydrobiopterin TPH expressed in Pichia pastoris Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0214
-
 TPH expressed in Pichia pastoris Homo sapiens
0.296
-
 TPH as fusion protein with maltose-binding protein expressed in Escherichia coli Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-tryptophan + (6R)-L-erytho-5,6,7,8-tetrahydrobiopterin + O2
-
 Homo sapiens 5-hydroxy-L-tryptophan + ?
-
 ?

Synonyms

Synonyms Comment Organism
TPH
-
 Homo sapiens