Cloned (Comment) | Organism |
---|---|
His-tagged enzyme, expression in Escherichia coli | Schistosoma mansoni |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-hydroxytryptophan | 0.018 mM, 50% inhibition | Schistosoma mansoni | |
D,L-4-chlorophenylalanine | 0.009 mM, 50% inhibition | Schistosoma mansoni | |
dopamine | 0.022 mM, 50% inhibition | Schistosoma mansoni |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0067 | - |
tetrahydrobiopterin | recombinant His-tagged enzyme | Schistosoma mansoni | |
0.022 | - |
L-tryptophan | recombinant His-tagged enzyme | Schistosoma mansoni |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
4 * 60000, His-tagged recombinant enzyme, immunoblot | Schistosoma mansoni |
240000 | - |
recombinant His-tagged enzyme, gel filtration | Schistosoma mansoni |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Schistosoma mansoni | O96370 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme, Nickel affinity chromatography | Schistosoma mansoni |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.17 | - |
recombinant His-tagged enzyme | Schistosoma mansoni |
Storage Stability | Organism |
---|---|
recombinant His-tagged enzyme, -80°C, 50 mM HEPES, pH 7.5, 200 mM NaCl, 10% glycerol, 0.05% Tween 20, 1 mM dithiothreitol, 4 days, no loss of activity | Schistosoma mansoni |
recombinant His-tagged enzyme, 4°C, 50 mM HEPES, pH 7.5, 200 mM NaCl, 10% glycerol, 0.05% Tween 20, 1 mM dithiothreitol, 4 days, no loss of activity | Schistosoma mansoni |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tryptophan + tetrahydrobiopterin + O2 | - |
Schistosoma mansoni | 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin | - |
r | |
L-tryptophan + tetrahydropteridine + O2 | - |
Oryctolagus cuniculus | 5-hydroxy-L-tryptophan + dihydropteridine + H2O | - |
? | |
L-tryptophan + tetrahydropteridine + O2 | - |
Schistosoma mansoni | 5-hydroxy-L-tryptophan + dihydropteridine + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | - |
Oryctolagus cuniculus |
tetramer | 4 * 60000, His-tagged recombinant enzyme, immunoblot | Schistosoma mansoni |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
half-life: 54 min | Oryctolagus cuniculus |
37 | - |
80 min, no loss of activity, estimated half-life: 21 h | Schistosoma mansoni |