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Literature summary for 1.14.16.4 extracted from

  • Hamdan, F.F.; Ribeiro, P.
    Characterization of a stable form of tryptophan hydroxylase from the human parasite Schistosoma mansoni (1999), J. Biol. Chem., 274, 21746-21754.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
His-tagged enzyme, expression in Escherichia coli Schistosoma mansoni

Inhibitors

Inhibitors Comment Organism Structure
5-hydroxytryptophan 0.018 mM, 50% inhibition Schistosoma mansoni
D,L-4-chlorophenylalanine 0.009 mM, 50% inhibition Schistosoma mansoni
dopamine 0.022 mM, 50% inhibition Schistosoma mansoni

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0067
-
tetrahydrobiopterin recombinant His-tagged enzyme Schistosoma mansoni
0.022
-
L-tryptophan recombinant His-tagged enzyme Schistosoma mansoni

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
4 * 60000, His-tagged recombinant enzyme, immunoblot Schistosoma mansoni
240000
-
recombinant His-tagged enzyme, gel filtration Schistosoma mansoni

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-
Schistosoma mansoni O96370
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme, Nickel affinity chromatography Schistosoma mansoni

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.17
-
recombinant His-tagged enzyme Schistosoma mansoni

Storage Stability

Storage Stability Organism
recombinant His-tagged enzyme, -80°C, 50 mM HEPES, pH 7.5, 200 mM NaCl, 10% glycerol, 0.05% Tween 20, 1 mM dithiothreitol, 4 days, no loss of activity Schistosoma mansoni
recombinant His-tagged enzyme, 4°C, 50 mM HEPES, pH 7.5, 200 mM NaCl, 10% glycerol, 0.05% Tween 20, 1 mM dithiothreitol, 4 days, no loss of activity Schistosoma mansoni

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-tryptophan + tetrahydrobiopterin + O2
-
Schistosoma mansoni 5-hydroxy-L-tryptophan + 4a-hydroxytetrahydrobiopterin
-
r
L-tryptophan + tetrahydropteridine + O2
-
Oryctolagus cuniculus 5-hydroxy-L-tryptophan + dihydropteridine + H2O
-
?
L-tryptophan + tetrahydropteridine + O2
-
Schistosoma mansoni 5-hydroxy-L-tryptophan + dihydropteridine + H2O
-
?

Subunits

Subunits Comment Organism
tetramer
-
Oryctolagus cuniculus
tetramer 4 * 60000, His-tagged recombinant enzyme, immunoblot Schistosoma mansoni

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
half-life: 54 min Oryctolagus cuniculus
37
-
80 min, no loss of activity, estimated half-life: 21 h Schistosoma mansoni