Activating Compound | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | 1 mM, 7% activation | Thunnus albacares |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CoCl2 | 1 mM, complete inhibition | Thunnus albacares | |
D,L-4-chlorophenylalanine | - |
Thunnus albacares | |
ZnSO4 | 1 mM, 80% inhibition | Thunnus albacares |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.023 | - |
L-tryptophan | - |
Thunnus albacares |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
96000 | - |
4 * 96000, SDS-PAGE | Thunnus albacares |
280000 | - |
liver enzyme, gel filtration | Thunnus albacares |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thunnus albacares | - |
sp. scombroidei, yellofin tuna | - |
Purification (Comment) | Organism |
---|---|
acid treatment, ammonium sulfate, Sepharose CL-6B, DEAE-sepharose, butyl-Sepharose, Toyopearl | Thunnus albacares |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Thunnus albacares | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
13.3 | - |
liver enzyme | Thunnus albacares |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tryptophan + tetrahydropteridine + O2 | - |
Thunnus albacares | 5-hydroxy-L-tryptophan + dihydropteridine + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 96000, SDS-PAGE | Thunnus albacares |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Thunnus albacares |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
stable below | Thunnus albacares |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Thunnus albacares |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | enzyme retains more than 80% activity after 60 min at 4°C between pH 7.0 and pH 7.6, unstable above pH 10.0 and below pH 5.0 | Thunnus albacares |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.021 | - |
D,L-4-chlorophenylalanine | - |
Thunnus albacares |