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Literature summary for 1.14.16.4 extracted from

  • Nagai, T.; Hamada, M.; Kai, N.; Tanoue, Y.; Nagayama, F.
    Characterization of yellowfin tuna (Thunnus albacares, Scombroidei) tryptophan hydroxylase (1997), Comp. Biochem. Physiol. B, 116, 161-165.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
CaCl2 1 mM, 7% activation Thunnus albacares

Inhibitors

Inhibitors Comment Organism Structure
CoCl2 1 mM, complete inhibition Thunnus albacares
D,L-4-chlorophenylalanine
-
Thunnus albacares
ZnSO4 1 mM, 80% inhibition Thunnus albacares

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.023
-
L-tryptophan
-
Thunnus albacares

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
96000
-
4 * 96000, SDS-PAGE Thunnus albacares
280000
-
liver enzyme, gel filtration Thunnus albacares

Organism

Organism UniProt Comment Textmining
Thunnus albacares
-
sp. scombroidei, yellofin tuna
-

Purification (Commentary)

Purification (Comment) Organism
acid treatment, ammonium sulfate, Sepharose CL-6B, DEAE-sepharose, butyl-Sepharose, Toyopearl Thunnus albacares

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Thunnus albacares
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
13.3
-
liver enzyme Thunnus albacares

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-tryptophan + tetrahydropteridine + O2
-
Thunnus albacares 5-hydroxy-L-tryptophan + dihydropteridine + H2O
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 96000, SDS-PAGE Thunnus albacares

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Thunnus albacares

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
stable below Thunnus albacares

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Thunnus albacares

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 10 enzyme retains more than 80% activity after 60 min at 4°C between pH 7.0 and pH 7.6, unstable above pH 10.0 and below pH 5.0 Thunnus albacares

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.021
-
D,L-4-chlorophenylalanine
-
Thunnus albacares