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Literature summary for 1.14.16.4 extracted from

  • Nakata, H.; Fujisawa, H.
    Purification and properties of tryptophan 5-monooxygenase from rat brain-stem (1982), Eur. J. Biochem., 122, 41-47.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.119
-
L-tryptophan
-
Rattus norvegicus
0.125
-
2-amino-4-hydroxy-6-methyltetrahydropteridine
-
Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ 0.02 mM, 3.5fold increase in activity Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59000
-
4 * 59000, tryptophan hydroxylase form I from brain stem, SDS-PAGE Rattus norvegicus
288000
-
-
Rattus norvegicus
300000
-
-
Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
brain stem Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.082
-
tryptophan hydroxylase form II Rattus norvegicus
0.374
-
-
Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-phenylalanine + tetrahydropteridine + O2 tryptophan hydroxylase form I, 39% of activity with L-tryptophan Rattus norvegicus tyrosine + dihydropteridine + H2O
-
?
L-tryptophan + 2-amino-4-hydroxy-6-methyl-tetrahydropteridine + O2
-
Rattus norvegicus ?
-
r
L-tryptophan + tetrahydropteridine + O2
-
Rattus norvegicus 5-hydroxy-L-tryptophan + dihydropteridine + H2O
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 59000, tryptophan hydroxylase form I from brain stem, SDS-PAGE Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
-
Rattus norvegicus

pH Range

pH Minimum pH Maximum Comment Organism
6 9.5 about 50% activity at pH 6 and 9.5 Rattus norvegicus