KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.119 | - |
L-tryptophan | - |
Rattus norvegicus | |
0.125 | - |
2-amino-4-hydroxy-6-methyltetrahydropteridine | - |
Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | 0.02 mM, 3.5fold increase in activity | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59000 | - |
4 * 59000, tryptophan hydroxylase form I from brain stem, SDS-PAGE | Rattus norvegicus |
288000 | - |
- |
Rattus norvegicus |
300000 | - |
- |
Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | stem | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.082 | - |
tryptophan hydroxylase form II | Rattus norvegicus |
0.374 | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-phenylalanine + tetrahydropteridine + O2 | tryptophan hydroxylase form I, 39% of activity with L-tryptophan | Rattus norvegicus | tyrosine + dihydropteridine + H2O | - |
? | |
L-tryptophan + 2-amino-4-hydroxy-6-methyl-tetrahydropteridine + O2 | - |
Rattus norvegicus | ? | - |
r | |
L-tryptophan + tetrahydropteridine + O2 | - |
Rattus norvegicus | 5-hydroxy-L-tryptophan + dihydropteridine + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 59000, tryptophan hydroxylase form I from brain stem, SDS-PAGE | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
- |
Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9.5 | about 50% activity at pH 6 and 9.5 | Rattus norvegicus |