Cloned (Comment) | Organism |
---|---|
- |
Bombyx mori |
Protein Variants | Comment | Organism |
---|---|---|
E434A | the mutant shows 35.6% of wild type activity. Furthermore, the mutation dramatically reduces its substrate affinity for tetrahydrobiopterin and decreases its activation by Fe2+ | Bombyx mori |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
guanidine hydrochloride | no activity at guanidine hydrochloride concentrations exceeding 0.6 M | Bombyx mori |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0263 | - |
L-tyrosine | recombinant wild type enzyme, at pH 7.5 and 37°C | Bombyx mori | |
0.029 | - |
L-tyrosine | native wild type enzyme, at pH 7.5 and 37°C | Bombyx mori | |
0.0428 | - |
L-tyrosine | mutant enzyme E434A, at pH 7.5 and 37°C | Bombyx mori | |
0.0471 | - |
tetrahydrobiopterin | native wild type enzyme, at pH 7.5 and 37°C | Bombyx mori | |
0.0519 | - |
tetrahydrobiopterin | recombinant wild type enzyme, at pH 7.5 and 37°C | Bombyx mori | |
0.341 | - |
tetrahydrobiopterin | mutant enzyme E434A, at pH 7.5 and 37°C | Bombyx mori |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | activates | Bombyx mori |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66200 | - |
x * 66200, SDS-PAGE | Bombyx mori |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tyrosine + tetrahydrobiopterin + O2 | Bombyx mori | - |
3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bombyx mori | - |
- |
- |
Purification (Comment) | Organism |
---|---|
heparin-Sepharose column chromatography, DEAE-Sepharose column chromatography, and Sephacryl S-200HR gel filtration | Bombyx mori |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Bombyx mori | - |
fat body | - |
Bombyx mori | - |
larva | - |
Bombyx mori | - |
nerve cord | - |
Bombyx mori | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tyrosine + tetrahydrobiopterin + O2 | - |
Bombyx mori | 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 66200, SDS-PAGE | Bombyx mori |
Synonyms | Comment | Organism |
---|---|---|
tyrosine hydroxylase | - |
Bombyx mori |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 65 | minimal aggregation occurs for wild type enzyme after heating at 40°C-45 for 60 min. A steep decrease in activity is observed between 40 and 65°C, and a complete loss of activity occurs at temperatures above 70°C | Bombyx mori |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
tetrahydrobiopterin | - |
Bombyx mori |