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Literature summary for 1.14.16.2 extracted from

  • Calvo, A.; Pey, A.; Miranda-Vizuete, A.; Døskeland, A.; Martinez, A.
    Divergence in enzyme regulation between Caenorhabditis elegans and human tyrosine hydroxylase, the key enzyme in the synthesis of dopamine (2011), Biochem. J., 434, 133-141.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) pLysS cells Homo sapiens
expressed in Escherichia coli BL21(DE3) pLysS cells Caenorhabditis elegans

Inhibitors

Inhibitors Comment Organism Structure
3,4-dihydroxy-L-phenylalanine product inhibition Caenorhabditis elegans
3,4-dihydroxy-L-phenylalanine product inhibition Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-tyrosine + tetrahydrobiopterin + O2 Homo sapiens the enzyme shows high substrate specificity for L-tyrosine 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O
-
?
L-tyrosine + tetrahydrobiopterin + O2 Caenorhabditis elegans the enzyme shows high substrate specificity for L-tyrosine 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O
-
?
L-tyrosine + tetrahydrobiopterin + O2 Caenorhabditis elegans N2 Bristol the enzyme shows high substrate specificity for L-tyrosine 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O
-
?

Organism

Organism UniProt Comment Textmining
Caenorhabditis elegans
-
-
-
Caenorhabditis elegans N2 Bristol
-
-
-
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein the enzyme is phosphorylated at Ser35 by cAMP-dependent protein kinase Caenorhabditis elegans
phosphoprotein the enzyme is phosphorylated at Ser40 by cAMP-dependent protein kinase Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
GST-Sepharose column chromatography Homo sapiens
GST-Sepharose column chromatography Caenorhabditis elegans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.555
-
with 0.05 mM L-tyrosine, at pH 7.0 and 25°C Homo sapiens
68800
-
with 1 mM L-tyrosine, at pH 7.0 and 25°C Caenorhabditis elegans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-tyrosine + tetrahydrobiopterin + O2 the enzyme shows high substrate specificity for L-tyrosine Homo sapiens 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O
-
?
L-tyrosine + tetrahydrobiopterin + O2 the enzyme shows high substrate specificity for L-tyrosine Caenorhabditis elegans 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O
-
?
L-tyrosine + tetrahydrobiopterin + O2 the enzyme shows high substrate specificity for L-tyrosine Caenorhabditis elegans N2 Bristol 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O
-
?

Synonyms

Synonyms Comment Organism
CAT-2
-
Caenorhabditis elegans
TH1
-
Homo sapiens
tyrosine hydroxylase
-
Homo sapiens
tyrosine hydroxylase
-
Caenorhabditis elegans

Cofactor

Cofactor Comment Organism Structure
tetrahydrobiopterin
-
Homo sapiens
tetrahydrobiopterin
-
Caenorhabditis elegans

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0022
-
at pH 7.0 and 25°C Homo sapiens 3,4-dihydroxy-L-phenylalanine
0.0326
-
at pH 7.0 and 25°C Caenorhabditis elegans 3,4-dihydroxy-L-phenylalanine

General Information

General Information Comment Organism
metabolism tyrosine hydroxylase is the key enzyme in the synthesis of dopamine Homo sapiens
metabolism tyrosine hydroxylase is the key enzyme in the synthesis of dopamine Caenorhabditis elegans