Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) pLysS cells | Homo sapiens |
expressed in Escherichia coli BL21(DE3) pLysS cells | Caenorhabditis elegans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3,4-dihydroxy-L-phenylalanine | product inhibition | Caenorhabditis elegans | |
3,4-dihydroxy-L-phenylalanine | product inhibition | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tyrosine + tetrahydrobiopterin + O2 | Homo sapiens | the enzyme shows high substrate specificity for L-tyrosine | 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O | - |
? | |
L-tyrosine + tetrahydrobiopterin + O2 | Caenorhabditis elegans | the enzyme shows high substrate specificity for L-tyrosine | 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O | - |
? | |
L-tyrosine + tetrahydrobiopterin + O2 | Caenorhabditis elegans N2 Bristol | the enzyme shows high substrate specificity for L-tyrosine | 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caenorhabditis elegans | - |
- |
- |
Caenorhabditis elegans N2 Bristol | - |
- |
- |
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | the enzyme is phosphorylated at Ser35 by cAMP-dependent protein kinase | Caenorhabditis elegans |
phosphoprotein | the enzyme is phosphorylated at Ser40 by cAMP-dependent protein kinase | Homo sapiens |
Purification (Comment) | Organism |
---|---|
GST-Sepharose column chromatography | Homo sapiens |
GST-Sepharose column chromatography | Caenorhabditis elegans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.555 | - |
with 0.05 mM L-tyrosine, at pH 7.0 and 25°C | Homo sapiens |
68800 | - |
with 1 mM L-tyrosine, at pH 7.0 and 25°C | Caenorhabditis elegans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tyrosine + tetrahydrobiopterin + O2 | the enzyme shows high substrate specificity for L-tyrosine | Homo sapiens | 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O | - |
? | |
L-tyrosine + tetrahydrobiopterin + O2 | the enzyme shows high substrate specificity for L-tyrosine | Caenorhabditis elegans | 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O | - |
? | |
L-tyrosine + tetrahydrobiopterin + O2 | the enzyme shows high substrate specificity for L-tyrosine | Caenorhabditis elegans N2 Bristol | 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CAT-2 | - |
Caenorhabditis elegans |
TH1 | - |
Homo sapiens |
tyrosine hydroxylase | - |
Homo sapiens |
tyrosine hydroxylase | - |
Caenorhabditis elegans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
tetrahydrobiopterin | - |
Homo sapiens | |
tetrahydrobiopterin | - |
Caenorhabditis elegans |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0022 | - |
at pH 7.0 and 25°C | Homo sapiens | 3,4-dihydroxy-L-phenylalanine | |
0.0326 | - |
at pH 7.0 and 25°C | Caenorhabditis elegans | 3,4-dihydroxy-L-phenylalanine |
General Information | Comment | Organism |
---|---|---|
metabolism | tyrosine hydroxylase is the key enzyme in the synthesis of dopamine | Homo sapiens |
metabolism | tyrosine hydroxylase is the key enzyme in the synthesis of dopamine | Caenorhabditis elegans |