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Literature summary for 1.14.16.2 extracted from

  • Wang, S.; Lasagna, M.; Daubner, S.C.; Reinhart, G.D.; Fitzpatrick, P.F.
    Fluorescence spectroscopy as a probe of the effect of phosphorylation at serine 40 of tyrosine hydroxylase on the conformation of its regulatory domain (2011), Biochemistry, 50, 2364-2370.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.16.2

Cloned(Commentary)

Cloned (Comment) Organism
-
 Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
W166F/W233F/W372F tryptophan-free enzyme with wild-type activity Rattus norvegicus
W166F/W233F/W372F/F14W introduced tryptophan residue in regulatory domain Rattus norvegicus
W166F/W233F/W372F/F34W introduced tryptophan residue in regulatory domain Rattus norvegicus
W166F/W233F/W372F/F74W introduced tryptophan residue in regulatory domain Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.024
-
 L-tyrosine W166F/W233F/W372F/F14W mutant protein, pH 7, 25°C Rattus norvegicus
0.04
-
 L-tyrosine wild type protein, pH 7, 25°C Rattus norvegicus
0.04
-
 tetrahydrobiopterin wild type protein, pH 7, 25°C Rattus norvegicus
0.043
-
 L-tyrosine W166F/W233F/W372F/F74W mutant protein, pH 7, 25°C Rattus norvegicus
0.055
-
 tetrahydrobiopterin W166F/W233F/W372F/F14W mutant protein, pH 7, 25°C Rattus norvegicus
0.058
-
 L-tyrosine W166F/W233F/W372F/F34W mutant protein, pH 7, 25°C Rattus norvegicus
0.065
-
 tetrahydrobiopterin W166F/W233F/W372F/F74W mutant protein, pH 7, 25°C Rattus norvegicus
0.077
-
 tetrahydrobiopterin W166F/W233F/W372F/F34W mutant protein, pH 7, 25°C Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+
-
 Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein phosphorylation of Ser40 activates the enzyme Rattus norvegicus

Purification (Commentary)

Purification (Comment) Organism
heparin Sepharose chromatography, incubation with ferrous iron, Q-Sepharose chromatography Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-tyrosine + tetrahydrobiopterin + O2
-
 Rattus norvegicus 3,4-dihydroxy-L-phenylalanine + 4a-hydroxytetrahydrobiopterin + H2O
-
 ?

Synonyms

Synonyms Comment Organism
tyrosine hydroxylase
-
 Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.7
-
 L-tyrosine W166F/W233F/W372F/F74W mutant protein, pH 7, 25°C Rattus norvegicus
1.8
-
 L-tyrosine W166F/W233F/W372F/F14W mutant protein, pH 7, 25°C Rattus norvegicus
2
-
 L-tyrosine W166F/W233F/W372F/F34W mutant protein, pH 7, 25°C Rattus norvegicus
2.5
-
 L-tyrosine wild type protein, pH 7, 25°C Rattus norvegicus