Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
A297L | outer edge of the active site | Homo sapiens |
E332D | active site residue, 10fold reduction in activity, close to the catalytic iron | Homo sapiens |
E332Q | active site residue, no activity, close to the catalytic iron | Homo sapiens |
F300A | active site residue | Homo sapiens |
F300Y | active site residue | Homo sapiens |
L294A | protrudes into catalytic cleft | Homo sapiens |
L294Y | protrudes into catalytic cleft | Homo sapiens |
Y371F | active site residue, close to the catalytic iron | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
dopamine | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0056 | - |
tetrahydrobiopterin | wild type, phosphorylated protein, pH 7.2, 30°C | Homo sapiens | |
0.0068 | - |
tetrahydrobiopterin | wild type protein, dopamine bound but removed from low-affinity site, pH 7.2, 30°C | Homo sapiens | |
0.0072 | - |
tetrahydrobiopterin | E332D phosphorylated mutant protein, pH 7.2, 30°C | Homo sapiens | |
0.0083 | - |
tetrahydrobiopterin | E332D mutant protein, pH 7.2, 30°C | Homo sapiens | |
0.0099 | - |
tetrahydrobiopterin | L294Y phosphorylated mutant protein, pH 7.2, 30°C | Homo sapiens | |
0.012 | - |
tetrahydrobiopterin | F300Y phosphorylated mutant protein, pH 7.2, 30°C | Homo sapiens | |
0.014 | - |
tetrahydrobiopterin | L294A mutant protein, pH 7.2, 30°C | Homo sapiens | |
0.014 | - |
tetrahydrobiopterin | wild type protein, pH 7.2, 30°C | Homo sapiens | |
0.015 | - |
tetrahydrobiopterin | L294Y mutant protein, pH 7.2, 30°C | Homo sapiens | |
0.017 | - |
tetrahydrobiopterin | A297L phosphorylated mutant protein, pH 7.2, 30°C | Homo sapiens | |
0.017 | - |
tetrahydrobiopterin | Y371F mutant protein, pH 7.2, 30°C | Homo sapiens | |
0.02 | - |
tetrahydrobiopterin | A297L mutant protein, pH 7.2, 30°C | Homo sapiens | |
0.026 | - |
tetrahydrobiopterin | F300Y mutant protein, pH 7.2, 30°C | Homo sapiens | |
0.027 | - |
tetrahydrobiopterin | A297L mutant protein, dopamine bound to the high-affinity site, pH 7.2, 30°C | Homo sapiens | |
0.027 | - |
tetrahydrobiopterin | Y371F mutant protein, dopamine bound to the high-affinity site, pH 7.2, 30°C | Homo sapiens | |
0.029 | - |
tetrahydrobiopterin | L294A phosphorylated mutant protein, pH 7.2, 30°C | Homo sapiens | |
0.029 | - |
tetrahydrobiopterin | Y371F phosphorylated mutant protein, pH 7.2, 30°C | Homo sapiens | |
0.034 | - |
tetrahydrobiopterin | E332D mutant protein, dopamine bound to the high-affinity site, pH 7.2, 30°C | Homo sapiens | |
0.054 | - |
tetrahydrobiopterin | F300A mutant protein, pH 7.2, 30°C | Homo sapiens | |
0.07 | - |
tetrahydrobiopterin | F300A phosphorylated mutant protein, pH 7.2, 30°C | Homo sapiens | |
0.134 | - |
tetrahydrobiopterin | wild type protein, dopamine bound to the high-affinity site, pH 7.2, 30°C | Homo sapiens | |
0.356 | - |
tetrahydrobiopterin | wild type protein, in the presence of dopamine, pH 7.2, 30°C | Homo sapiens | |
0.507 | - |
tetrahydrobiopterin | wild type, phosphorylated protein, in the presence of dopamine, pH 7.2, 30°C | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | - |
Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P07101 | - |
- |
Purification (Comment) | Organism |
---|---|
heparin Sepharose chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tyrosine + tetrahydrobiopterin + O2 | - |
Homo sapiens | 3,4-dihydroxy-L-phenylalanine + 4a-hydroxytetrahydrobiopterin + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
tyrosine hydroxylase | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
tetrahydrobiopterin | wild type protein, in the presence of 0.01 mM dopamine, pH 7.2, 30°C | Homo sapiens | |
0.047 | - |
tetrahydrobiopterin | E332D phosphorylated mutant protein, pH 7.2, 30°C | Homo sapiens | |
0.06 | - |
tetrahydrobiopterin | E332D mutant protein, pH 7.2, 30°C | Homo sapiens | |
0.068 | - |
tetrahydrobiopterin | L294Y phosphorylated mutant protein, pH 7.2, 30°C | Homo sapiens | |
0.088 | - |
tetrahydrobiopterin | F300A mutant protein, pH 7.2, 30°C | Homo sapiens | |
0.11 | - |
tetrahydrobiopterin | F300A phosphorylated mutant protein, pH 7.2, 30°C | Homo sapiens | |
0.12 | - |
tetrahydrobiopterin | L294Y mutant protein, pH 7.2, 30°C | Homo sapiens | |
0.14 | - |
tetrahydrobiopterin | L294A phosphorylated mutant protein, pH 7.2, 30°C | Homo sapiens | |
0.18 | - |
tetrahydrobiopterin | L294A mutant protein, pH 7.2, 30°C | Homo sapiens | |
0.2 | - |
tetrahydrobiopterin | Y371F mutant protein, pH 7.2, 30°C | Homo sapiens | |
0.22 | - |
tetrahydrobiopterin | A297L phosphorylated mutant protein, pH 7.2, 30°C | Homo sapiens | |
0.27 | - |
tetrahydrobiopterin | A297L mutant protein, pH 7.2, 30°C | Homo sapiens | |
0.27 | - |
tetrahydrobiopterin | F300Y phosphorylated mutant protein, pH 7.2, 30°C | Homo sapiens | |
0.3 | - |
tetrahydrobiopterin | F300Y mutant protein, pH 7.2, 30°C | Homo sapiens | |
0.3 | - |
tetrahydrobiopterin | Y371F phosphorylated mutant protein, pH 7.2, 30°C | Homo sapiens | |
0.47 | - |
tetrahydrobiopterin | wild type, phosphorylated protein, pH 7.2, 30°C | Homo sapiens | |
0.58 | - |
tetrahydrobiopterin | wild type protein, pH 7.2, 30°C | Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.000256 | - |
wild type, phosphorylated protein, pH 7.2, 30°C | Homo sapiens | dopamine | |
0.0004 | - |
wild type protein, pH 7.2, 30°C | Homo sapiens | dopamine | |
0.00093 | - |
F300Y mutant protein, pH 7.2, 30°C | Homo sapiens | dopamine | |
0.00098 | - |
Y371F mutant protein, pH 7.2, 30°C | Homo sapiens | dopamine | |
0.00107 | - |
L294A mutant protein, pH 7.2, 30°C | Homo sapiens | dopamine | |
0.00143 | - |
E332D mutant protein, pH 7.2, 30°C | Homo sapiens | dopamine | |
0.00248 | - |
E332D phosphorylated mutant protein, pH 7.2, 30°C | Homo sapiens | dopamine | |
0.0136 | - |
E332D phosphorylated mutant protein, pH 7.2, 30°C | Homo sapiens | dopamine |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.7 | - |
tetrahydrobiopterin | F300A mutant protein, pH 7.2, 30°C | Homo sapiens | |
1.7 | - |
tetrahydrobiopterin | F300A phosphorylated mutant protein, pH 7.2, 30°C | Homo sapiens | |
5 | - |
tetrahydrobiopterin | L294A phosphorylated mutant protein, pH 7.2, 30°C | Homo sapiens | |
6.5 | - |
tetrahydrobiopterin | E332D phosphorylated mutant protein, pH 7.2, 30°C | Homo sapiens | |
7 | - |
tetrahydrobiopterin | L294Y phosphorylated mutant protein, pH 7.2, 30°C | Homo sapiens | |
7.3 | - |
tetrahydrobiopterin | E332D mutant protein, pH 7.2, 30°C | Homo sapiens | |
7.7 | - |
tetrahydrobiopterin | L294Y mutant protein, pH 7.2, 30°C | Homo sapiens | |
10.7 | - |
tetrahydrobiopterin | Y371F phosphorylated mutant protein, pH 7.2, 30°C | Homo sapiens | |
11.2 | - |
tetrahydrobiopterin | F300Y mutant protein, pH 7.2, 30°C | Homo sapiens | |
11.7 | - |
tetrahydrobiopterin | Y371F mutant protein, pH 7.2, 30°C | Homo sapiens | |
12.5 | - |
tetrahydrobiopterin | A297L phosphorylated mutant protein, pH 7.2, 30°C | Homo sapiens | |
13 | - |
tetrahydrobiopterin | L294A mutant protein, pH 7.2, 30°C | Homo sapiens | |
13.7 | - |
tetrahydrobiopterin | A297L mutant protein, pH 7.2, 30°C | Homo sapiens | |
21.8 | - |
tetrahydrobiopterin | F300Y phosphorylated mutant protein, pH 7.2, 30°C | Homo sapiens | |
45.7 | - |
tetrahydrobiopterin | wild type protein, pH 7.2, 30°C | Homo sapiens | |
89.3 | - |
tetrahydrobiopterin | wild type, phosphorylated protein, pH 7.2, 30°C | Homo sapiens |