Literature summary for 1.14.16.2 extracted from

Gordon, S.L.; Webb, J.K.; Shehadeh, J.; Dunkley, P.R.; Dickson, P.W.
The low affinity dopamine binding site on tyrosine hydroxylase: the role of the N-terminus and in situ regulation of enzyme activity (2009), Neurochem. Res., 34, 1830-1837.

Search for more literature for 1.14.16.2

Activating Compound

Activating Compound	Comment	Organism	Structure
forskolin	forskolin selectively increases the phosphorylation of tyrosine hydroxylase at Ser40 by 1.8fold without increasing total tyrosine hydroxylase protein levels	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
additional information	deletion of the N-terminus of tyrosine hydroxylase removes the high affinity dopamine binding site, but does not affect dopamine binding to the low affinity site	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
3,4-dihydroxy-L-phenylalanine	incubation with 3,4-dihydroxy-L-phenylalanine results in an inhibition of tyrosine hydroxylase activity in situ under both basal conditions and conditions that promote the phosphorylation of Ser40. 3,4-dihydroxy-L-phenylalanine binding to the high affinity site alone inhibits tyrosine hydroxylase activity to approximately 15% and 24% of control (non-dopamine bound) levels at 0.02 mM and 2.0 mM 5,6,7,8-tetrahydrobiopterin, respectively	Homo sapiens
additional information	there is no significant decrease in tyrosine hydroxylase activity at 0.02 mM or 2.0 mM 5,6,7,8-tetrahydrobiopterin following ovalbumin-coated charcoal treatment	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-chelating Sepharose fast flow bead chromatography	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
L-tyrosine + 5,6,7,8,-tetrahydro-L-biopterin + O2	-	Homo sapiens	3,4-dihydroxy-L-phenylalanine + 4a-hydroxytetrahydro-L-biopterin	-	?

Synonyms

Synonyms	Comment	Organism
TH1	isozyme	Homo sapiens
tyrosine hydroxylase	-	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
5,6,7,8-tetrahydro-L-biopterin	-	Homo sapiens

General Information

General Information	Comment	Organism
metabolism	tyrosine hydroxylase is the rate-limiting enzyme in catecholamine biosynthesis	Homo sapiens

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Release 2023.1 (January 2023)