Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain XL-1 Blue | Manduca sexta |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63100 | - |
4 * 63100, about, recombinant enzyme, sequence calculation and gel filtration | Manduca sexta |
250000 | - |
about, recombinant enzyme, gel filtration | Manduca sexta |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tyrosine + tetrahydrobiopterin + O2 | Manduca sexta | the enzyme is a major producer of the DOPA required for both cuticle tanning and immune-associated melanization, overview | 3,4-dihydroxy-L-phenylalanine + 4a-hydroxytetrahydrobiopterin + H2O | i.e. L-dopa | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Manduca sexta | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | the enzyme is phosphorylated by cAMP-dependent protein kinases at serine residues, e.g. Ser31 | Manduca sexta |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain XL-1 Blue by nickel affinity chromatography and gel filtration | Manduca sexta |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
egg | enzyme expression at the stage when the pharate larval cuticle begins to tan | Manduca sexta | - |
larva | enzyme expression in the integument of molting larvae | Manduca sexta | - |
additional information | tissue enzyme expression profile, overview | Manduca sexta | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tyrosine + tetrahydrobiopterin + O2 | - |
Manduca sexta | 3,4-dihydroxy-L-phenylalanine + 4a-hydroxytetrahydrobiopterin + H2O | - |
? | |
L-tyrosine + tetrahydrobiopterin + O2 | the enzyme is a major producer of the DOPA required for both cuticle tanning and immune-associated melanization, overview | Manduca sexta | 3,4-dihydroxy-L-phenylalanine + 4a-hydroxytetrahydrobiopterin + H2O | i.e. L-dopa | ? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 63100, about, recombinant enzyme, sequence calculation and gel filtration | Manduca sexta |
Synonyms | Comment | Organism |
---|---|---|
tyrosine hydroxylase | - |
Manduca sexta |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
tetrahydrobiopterin | - |
Manduca sexta |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Manduca sexta | sequence calculation | - |
5.43 |