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Literature summary for 1.14.16.2 extracted from
- Characterization of tyrosine hydroxylase from Manduca sexta (2007), Insect Biochem. Mol. Biol., 37, 1327-1337.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain XL-1 Blue | Manduca sexta |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 63100 | - |
4 * 63100, about, recombinant enzyme, sequence calculation and gel filtration | Manduca sexta |
| 250000 | - |
about, recombinant enzyme, gel filtration | Manduca sexta |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-tyrosine + tetrahydrobiopterin + O2 | Manduca sexta | the enzyme is a major producer of the DOPA required for both cuticle tanning and immune-associated melanization, overview | 3,4-dihydroxy-L-phenylalanine + 4a-hydroxytetrahydrobiopterin + H2O | i.e. L-dopa | ? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Manduca sexta | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phosphoprotein | the enzyme is phosphorylated by cAMP-dependent protein kinases at serine residues, e.g. Ser31 | Manduca sexta |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain XL-1 Blue by nickel affinity chromatography and gel filtration | Manduca sexta |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| egg | enzyme expression at the stage when the pharate larval cuticle begins to tan | Manduca sexta | - |
| larva | enzyme expression in the integument of molting larvae | Manduca sexta | - |
| additional information | tissue enzyme expression profile, overview | Manduca sexta | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| L-tyrosine + tetrahydrobiopterin + O2 | - |
Manduca sexta | 3,4-dihydroxy-L-phenylalanine + 4a-hydroxytetrahydrobiopterin + H2O | - |
? | |
| L-tyrosine + tetrahydrobiopterin + O2 | the enzyme is a major producer of the DOPA required for both cuticle tanning and immune-associated melanization, overview | Manduca sexta | 3,4-dihydroxy-L-phenylalanine + 4a-hydroxytetrahydrobiopterin + H2O | i.e. L-dopa | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 63100, about, recombinant enzyme, sequence calculation and gel filtration | Manduca sexta |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| tyrosine hydroxylase | - |
Manduca sexta |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| tetrahydrobiopterin | - |
Manduca sexta | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Manduca sexta | sequence calculation | - |
5.43 |
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