Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.14.16.2 extracted from

  • Sura, G.R.; Lasagna, M.; Gawandi, V.; Reinhart, G.D.; Fitzpatrick, P.F.
    Effects of ligands on the mobility of an active-site loop in tyrosine hydroxylase as monitored by fluorescence anisotropy (2006), Biochemistry, 45, 9632-9638.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21(DE3) Homo sapiens

Protein Variants

Protein Variants Comment Organism
F184W/W372F 4fold lower Km for L-tyrosine compared to the wild-type enzyme. Similar Km for 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine compared to wild-type enzyme Homo sapiens
W166F/F184W/W233F/W372F mutant protein contains one tryptophan at residue 184 in the middle of a mobile active-site loop. The mutant was generated to perform steady-state fluorescence anisotropy measurements and shows kinetic properties similar to the wild-type enzyme Homo sapiens
W166F/F184W/W372F Km for L-tyrosine similar to the wild-type enzyme. 3fold higher Km for 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine compared to wild-type enzyme Homo sapiens
W166F/W233F/W372F Km for L-tyrosine similar to the wild-type enzyme. 4fold higher Km for 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine compared to wild-type enzyme Homo sapiens
W166F/W372F Km for L-tyrosine similar to the wild-type enzyme. 3fold higher Km for 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine compared to wild-type enzyme Homo sapiens
W372F 10fold lower Km for L-tyrosine compared to the wild-type enzyme. Similar Km for 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine compared to wild-type enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.045
-
6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine 0.1 mM L-tyrosine, 0.1 mg/ml catalase, 50 mM HEPES pH 7.0, 0.01 mM ferrous ammonium sulfate, 1 mM dithiothreitol, 25°C Homo sapiens
0.08
-
L-tyrosine 0.38 mM 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine, 0.1 mg/ml catalase, 50 mM HEPES pH 7.0, 0.01 mM ferrous ammonium sulfate, 1 mM dithiothreitol, 25°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Hi-TRAP heparin column Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-tyrosine + 6,7-dimethyl-2-amino-4-hydroxy-5,6,7,8-tetrahydopteridine + O2
-
Homo sapiens 3,4-dihydroxyphenylalanine + ?
-
?

Synonyms

Synonyms Comment Organism
TyrH
-
Homo sapiens
tyrosine hydroxylase
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.03
-
L-tyrosine 0.1 mM L-tyrosine, 0.1 mg/ml catalase, 50 mM HEPES pH 7.0, 0.01 mM ferrous ammonium sulfate, 1 mM dithiothreitol, 25°C Homo sapiens