Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
R306H | 1.2fold decrease in Km-value for L-tyrosine compared to wild-type enzyme, Ki-value for L-tyrosine is nearly identical to wild-type value, 1.3fold decrease in KM-value for tetrahydrobiopterin compared to wild-type enzyme, 1.2fold increase of turnover-number compared to wild-type enzyme. 8.2°C increase in Tm-value compared to wild-type enzyme | Homo sapiens |
R306H/T463M | all of the TyrH is insoluble and no enzyme can be purified | Homo sapiens |
T245P | 1.4fold increase in Km-value for L-tyrosine compared to wild-type enzyme, 1.6fold increase in Ki-value for L-tyrosine compared to wild-type enzyme, 1.1fold increase in KM-value for tetrahydrobiopterin compared to wild-type enzyme, 1.6fold increase of turnover-number compared to wild-type enzyme. 3.9°C increase in Tm-value compared to wild-type enzyme | Homo sapiens |
T245P/T283M | all of the TyrH is insoluble and no enzyme can be purified | Homo sapiens |
T283M | 1.2fold decrease in Km-value for L-tyrosine compared to wild-type enzyme, 1.2fold decrease in Ki-value for L-tyrosine compared to wild-type enzyme, 1.3fold decrease in KM-value for tetrahydrobiopterin compared to wild-type enzyme, 4.2fold decrease of turnover-number compared to wild-type enzyme | Homo sapiens |
T463M | 1.1fold decrease in Km-value for L-tyrosine compared to wild-type enzyme, Ki-value for L-tyrosine is nearly identical to wild-type value compared to wild-type enzyme, 1.4fold decrease in KM-value for tetrahydrobiopterin compared to wild-type enzyme, 1.2fold increase of turnover-number compared to wild-type enzyme. 7.7°C increase in Tm-value compared to wild-type enzyme | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-tyrosine | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
tetrahydrobiopterin | pH 7.0, 30°C, mutant enzyme T463M | Homo sapiens | |
0.01 | - |
tetrahydrobiopterin | pH 7.0, 30°C, mutant enzyme R306H | Homo sapiens | |
0.01 | - |
tetrahydrobiopterin | pH 7.0, 30°C, mutant enzyme T283M | Homo sapiens | |
0.013 | - |
tetrahydrobiopterin | pH 7.0, 30°C, wild-type enzyme | Homo sapiens | |
0.014 | - |
tetrahydrobiopterin | pH 7.0, 30°C, mutant enzyme T245P | Homo sapiens | |
0.039 | - |
L-tyrosine | pH 7.0, 30°C, mutant enzyme T283M | Homo sapiens | |
0.042 | - |
L-tyrosine | pH 7.0, 30°C, mutant enzyme R306H | Homo sapiens | |
0.043 | - |
L-tyrosine | pH 7.0, 30°C, mutant enzyme T463M | Homo sapiens | |
0.046 | - |
L-tyrosine | pH 7.0, 30°C, wild-type enzyme | Homo sapiens | |
0.066 | - |
L-tyrosine | pH 7.0, 30°C, mutant enzyme T245P | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
inclusion body | mutant enzymes T283M and R306H expressed in Escherichia coli are mainly found in inclusion bodies but a small amount of soluble enzyme is produced | Homo sapiens | 16234 | - |
soluble | mutant enzyme T245P and T463 M expressed in Escherichia coli are predominantly soluble. Mutant enzymes T283M and R306H expressed in Escherichia coli are mainly found in inclusion bodies but a small amount of soluble enzyme is produced | Homo sapiens | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | the enzyme catalyzes the rate-limiting step in the biosynthesis of dopmaine. The physiological effects of the mutations described in cases of autosomal recessive DOPA-responsive dystonia are primarily due to the decreased stability of the mutant proteins razher than decreases in their intrinsic activities | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Storage Stability | Organism |
---|---|
-80°C, purified wild-type enzyme can be stored indefinetely in absence of glycerol. the mutant enzymes T245P and T463M behave similarly. The removal of glyceryl from the T283M and R306H enzymes results in significant precipitation and loss of the protein | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tyrosine + tetrahydrobiopterin + O2 | - |
Homo sapiens | 3,4-dihydroxy-L-phenylalanine + 4a-hydroxytetrahydrobiopterin + H2O | - |
? | |
additional information | the enzyme catalyzes the rate-limiting step in the biosynthesis of dopmaine. The physiological effects of the mutations described in cases of autosomal recessive DOPA-responsive dystonia are primarily due to the decreased stability of the mutant proteins razher than decreases in their intrinsic activities | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TyrH | - |
Homo sapiens |
tyrosine hydroxylase | - |
Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
41 | - |
Tm-value of mutant enzyme T463M is 44.3°C | Homo sapiens |
44 | - |
Tm-value of mutant enzyme T245P is 44.3°C | Homo sapiens |
46 | - |
Tm-value of mutant enzyme R306H is 45.8°C | Homo sapiens |
48 | - |
Tm-value of wild-type enzyme is 48.2°C | Homo sapiens |
50 | - |
50% loss of activity after 90 min, 80% loss of activity after 2 h, wild-type enzyme | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.8 | - |
L-tyrosine | pH 7.0, 30°C, mutant enzyme T283M | Homo sapiens | |
3.33 | - |
L-tyrosine | pH 7.0, 30°C, wild-type enzyme | Homo sapiens | |
3.68 | - |
L-tyrosine | pH 7.0, 30°C, mutant enzyme R306H | Homo sapiens | |
3.87 | - |
L-tyrosine | pH 7.0, 30°C, mutant enzyme T463M | Homo sapiens | |
5.35 | - |
L-tyrosine | pH 7.0, 30°C, mutant enzyme T245P | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
tetrahydrobiopterin | - |
Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.037 | - |
L-tyrosine | pH 7.0, 30°C, mutant enzyme T283M | Homo sapiens | |
0.044 | - |
L-tyrosine | pH 7.0, 30°C, mutant enzyme T463M | Homo sapiens | |
0.046 | - |
L-tyrosine | pH 7.0, 30°C, wild-type enzyme | Homo sapiens | |
0.048 | - |
L-tyrosine | pH 7.0, 30°C, mutant enzyme R306H | Homo sapiens | |
0.073 | - |
L-tyrosine | pH 7.0, 30°C, mutant enzyme T245P | Homo sapiens |