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Literature summary for 1.14.16.2 extracted from

  • Royo, M.; Fitzpatrick, P.F.; Daubner, S.C.
    Mutation of regulatory serines of rat tyrosine hydroxylase to glutamate: effects on enzyme stability and activity (2005), Arch. Biochem. Biophys., 434, 266-274.
    View publication on PubMed
Search for more literature for 1.14.16.2

Protein Variants

Protein Variants Comment Organism
S19E similar steady-state parameters and similar binding affinity for catecholamines to wild-type enzyme. Inactivated 1.8fold slower than the wild-type enzyme at 42°C in presence of 2% glycerol Rattus norvegicus
S31E similar steady-state parameters and similar binding affinity for catecholamines to wild-type enzyme. Inactivated 1.8fold slower than the wild-type enzyme at 42°C in presence of 2% glycerol Rattus norvegicus
S40E inactivated 1.6fold faster than the wild-type enzyme at 42°C in presence of 2% glycerol Rattus norvegicus
S8E similar steady-state parameters and similar binding affinity for catecholamines to wild-type enzyme. More stable than wild-type enzyme at 42°C in presence of 2% glycerol Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005
-
 L-tyrosine pH 7.0, 30°C, mutant enzyme S31A, cosubstrate 0.3 mM tetrahydropterin Rattus norvegicus
0.008
-
 L-tyrosine pH 7.0, 30°C, mutant enzyme S31E, cosubstrate 0.3 mM tetrahydropterin Rattus norvegicus
0.009
-
 L-tyrosine pH 7.0, 30°C, mutant enzyme S19A, cosubstrate 0.3 mM tetrahydropterin Rattus norvegicus
0.009
-
 L-tyrosine pH 7.0, 30°C, mutant enzyme S19E, cosubstrate 0.3 mM tetrahydropterin Rattus norvegicus
0.009
-
 L-tyrosine pH 7.0, 30°C, mutant enzyme S19E/S40E, cosubstrate 0.3 mM tetrahydropterin Rattus norvegicus
0.009
-
 L-tyrosine pH 7.0, 30°C, mutant enzyme S40E, cosubstrate 0.3 mM tetrahydropterin Rattus norvegicus
0.009
-
 L-tyrosine pH 7.0, 30°C, wild-type enzyme, cosubstrate 0.3 mM tetrahydropterin Rattus norvegicus
0.01
-
 L-tyrosine pH 7.0, 30°C, mutant enzyme S40A, cosubstrate 0.3 mM tetrahydropterin Rattus norvegicus
0.011
-
 L-tyrosine pH 7.0, 30°C, mutant enzyme S8A, cosubstrate 0.3 mM tetrahydropterin Rattus norvegicus
0.011
-
 L-tyrosine pH 7.0, 30°C, mutant enzyme S8E, cosubstrate 0.3 mM tetrahydropterin Rattus norvegicus
0.023
-
 (RS)-6-methyl-5,6,7,8-tetrahydropterin pH 7.0, 30°C, mutant enzyme S19E/S40E Rattus norvegicus
0.026
-
 L-tyrosine pH 7.0, 30°C, mutant enzyme S19E, cosubstrate 0.4 mM 6-methyltetrahydropterin Rattus norvegicus
0.028
-
 (RS)-6-methyl-5,6,7,8-tetrahydropterin pH 7.0, 30°C, mutant enzyme S8E Rattus norvegicus
0.032
-
 (RS)-6-methyl-5,6,7,8-tetrahydropterin pH 7.0, 30°C, mutant enzyme S19A Rattus norvegicus
0.033
-
 (RS)-6-methyl-5,6,7,8-tetrahydropterin pH 7.0, 30°C, mutant enzyme S19A Rattus norvegicus
0.033
-
 (RS)-6-methyl-5,6,7,8-tetrahydropterin pH 7.0, 30°C, mutant enzyme S19E Rattus norvegicus
0.034
-
 (RS)-6-methyl-5,6,7,8-tetrahydropterin pH 7.0, 30°C, wild-type enzyme Rattus norvegicus
0.035
-
 L-tyrosine pH 7.0, 30°C, mutant enzyme S19A, cosubstrate 0.4 mM 6-methyltetrahydropterin Rattus norvegicus
0.035
-
 (RS)-6-methyl-5,6,7,8-tetrahydropterin pH 7.0, 30°C, mutant enzyme S40A Rattus norvegicus
0.035
-
 (RS)-6-methyl-5,6,7,8-tetrahydropterin pH 7.0, 30°C, mutant enzyme S40E Rattus norvegicus
0.035
-
 (RS)-6-methyl-5,6,7,8-tetrahydropterin pH 7.0, 30°C, mutant enzyme S8E Rattus norvegicus
0.036
-
 (RS)-6-methyl-5,6,7,8-tetrahydropterin pH 7.0, 30°C, mutant enzyme S8A Rattus norvegicus
0.037
-
 (RS)-6-methyl-5,6,7,8-tetrahydropterin pH 7.0, 30°C, mutant enzyme S19E Rattus norvegicus
0.037
-
 (RS)-6-methyl-5,6,7,8-tetrahydropterin pH 7.0, 30°C, mutant enzyme S19E/S40E Rattus norvegicus
0.037
-
 (RS)-6-methyl-5,6,7,8-tetrahydropterin pH 7.0, 30°C, mutant enzyme S40E Rattus norvegicus
0.039
-
 (RS)-6-methyl-5,6,7,8-tetrahydropterin pH 7.0, 30°C, mutant enzyme S8A Rattus norvegicus
0.04
-
 (RS)-6-methyl-5,6,7,8-tetrahydropterin pH 7.0, 30°C, wild-type enzyme Rattus norvegicus
0.041
-
 L-tyrosine pH 7.0, 30°C, wild-type enzyme, cosubstrate 0.4 mM 6-methyltetrahydropterin Rattus norvegicus
0.042
-
 L-tyrosine pH 7.0, 30°C, mutant enzyme S8A, cosubstrate 0.4 mM 6-methyltetrahydropterin Rattus norvegicus
0.045
-
 (RS)-6-methyl-5,6,7,8-tetrahydropterin pH 7.0, 30°C, mutant enzyme S40A Rattus norvegicus
0.046
-
 L-tyrosine pH 7.0, 30°C, mutant enzyme S40A, cosubstrate 0.4 mM 6-methyltetrahydropterin Rattus norvegicus
0.047
-
 (RS)-6-methyl-5,6,7,8-tetrahydropterin pH 7.0, 30°C, mutant enzyme S31E Rattus norvegicus
0.047
-
 L-tyrosine pH 7.0, 30°C, mutant enzyme S40E, cosubstrate 0.4 mM 6-methyltetrahydropterin Rattus norvegicus
0.047
-
 L-tyrosine pH 7.0, 30°C, mutant enzyme S8E, cosubstrate 0.4 mM 6-methyltetrahydropterin Rattus norvegicus
0.048
-
 (RS)-6-methyl-5,6,7,8-tetrahydropterin pH 7.0, 30°C, mutant enzyme S31A Rattus norvegicus
0.051
-
 (RS)-6-methyl-5,6,7,8-tetrahydropterin pH 7.0, 30°C, mutant enzyme S31A Rattus norvegicus
0.051
-
 (RS)-6-methyl-5,6,7,8-tetrahydropterin pH 7.0, 30°C, mutant enzyme S31E Rattus norvegicus
0.053
-
 L-tyrosine pH 7.0, 30°C, mutant enzyme S31E, cosubstrate 0.4 mM 6-methyltetrahydropterin Rattus norvegicus
0.055
-
 L-tyrosine pH 7.0, 30°C, mutant enzyme S31A, cosubstrate 0.4 mM 6-0.053 methyltetrahydropterin Rattus norvegicus
0.059
-
 L-tyrosine pH 7.0, 30°C, mutant enzyme S19E/S40E, cosubstrate 0.4 mM 6-methyltetrahydropterin Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-tyrosine + 6-methyltetrahydropterin + O2
-
 Rattus norvegicus ?
-
 ?
L-tyrosine + tetrahydropterin + O2
-
 Rattus norvegicus ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
42
-
 or below, all mutant enzymes are stable for at least 1 h Rattus norvegicus
45
-
 in presence of 2% glycerol, enzymes lose activity in time-dependent manner Rattus norvegicus
50
-
 in presence of 10% glycerol, all mutant enzymes are stable for at least 1 h Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
(RS)-6-methyl-5,6,7,8-tetrahydropterin
-
 Rattus norvegicus
tetrahydrobiopterin
-
 Rattus norvegicus