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Literature summary for 1.14.16.2 extracted from

  • Kaufman, S.
    Aromatic amino acid hydroxylases (1987), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 18, 217-282.
No PubMed abstract available

General Stability

General Stability Organism
phosphorylated enzyme is less stable than nonphosphorylated form Rattus norvegicus
phosphorylated enzyme is less stable than nonphosphorylated form Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
L-Dopa
-
Bos taurus
L-Dopa
-
Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Rattus norvegicus
additional information
-
additional information
-
Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
peripheral noradrenergic neurons in superior cervical ganglion: MW 130000 Da Rattus norvegicus
additional information
-
peripheral noradrenergic neurons in superior cervical ganglion: MW 130000 Da Bos taurus
additional information
-
high molecular weight of brain enzyme is partly due to association with RNA. This makes it difficult to decide whether tyrosine hydroxylase molecules of different structure are present in the various regions of the brain, cell bodies of noradrenergic neurons: MW 200000 Da, substancia nigra and caudate nucleus, dopaminergic neurons: MW 65000 Da Rattus norvegicus
additional information
-
high molecular weight of brain enzyme is partly due to association with RNA. This makes it difficult to decide whether tyrosine hydroxylase molecules of different structure are present in the various regions of the brain, cell bodies of noradrenergic neurons: MW 200000 Da, substancia nigra and caudate nucleus, dopaminergic neurons: MW 65000 Da Bos taurus
59000
-
4 * 59000, SDS-PAGE Rattus norvegicus
60000
-
4 * 60000, SDS-PAGE Rattus norvegicus
60000
-
4 * 60000, SDS-PAGE Bos taurus
62000
-
4 * 62000 Rattus norvegicus
260000
-
from adrenal medulla Rattus norvegicus
280000
-
-
Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-tyrosine + (6R)-L-erythro-1',2'-dihydroxypropyltetrahydropterin + O2 Rattus norvegicus first step in biosynthesis of catecholamines such as norepinephrine, epinephrine and dopamine ?
-
?
L-tyrosine + (6R)-L-erythro-1',2'-dihydroxypropyltetrahydropterin + O2 Bos taurus first step in biosynthesis of catecholamines such as norepinephrine, epinephrine and dopamine ?
-
?
L-tyrosine + tetrahydrobiopterin + O2 Rattus norvegicus first step in biosynthesis of catecholamines such as norepinephrine, epinephrine and dopamine 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O
-
?
L-tyrosine + tetrahydrobiopterin + O2 Bos taurus first step in biosynthesis of catecholamines such as norepinephrine, epinephrine and dopamine 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O
-
?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Rattus norvegicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
adrenal medulla
-
Rattus norvegicus
-
adrenal medulla
-
Bos taurus
-
brain
-
Rattus norvegicus
-
brain
-
Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-tyrosine + (6R)-L-erythro-1',2'-dihydroxypropyltetrahydropterin + O2 first step in biosynthesis of catecholamines such as norepinephrine, epinephrine and dopamine Rattus norvegicus ?
-
?
L-tyrosine + (6R)-L-erythro-1',2'-dihydroxypropyltetrahydropterin + O2 first step in biosynthesis of catecholamines such as norepinephrine, epinephrine and dopamine Bos taurus ?
-
?
L-tyrosine + tetrahydrobiopterin + O2
-
Rattus norvegicus 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O 3,4-dihydroxy-L-phenylalanine is identical with dopa ?
L-tyrosine + tetrahydrobiopterin + O2
-
Bos taurus 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O 3,4-dihydroxy-L-phenylalanine is identical with dopa ?
L-tyrosine + tetrahydrobiopterin + O2 first step in biosynthesis of catecholamines such as norepinephrine, epinephrine and dopamine Rattus norvegicus 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O
-
?
L-tyrosine + tetrahydrobiopterin + O2 first step in biosynthesis of catecholamines such as norepinephrine, epinephrine and dopamine Bos taurus 3,4-dihydroxy-L-phenylalanine + dihydrobiopterin + H2O
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 62000 Rattus norvegicus
tetramer 4 * 60000, SDS-PAGE Rattus norvegicus
tetramer 4 * 60000, SDS-PAGE Bos taurus
tetramer 4 * 59000, SDS-PAGE Rattus norvegicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
half-life of activated, phosphorylated enzyme: 5 min, half-life of nonphosphorylated enzyme: 15 min Rattus norvegicus
50
-
half-life of activated, phosphorylated enzyme: 5 min, half-life of nonphosphorylated enzyme: 15 min Bos taurus