Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli TB1 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
Y138A | the mutant shows reduced catalytic efficiency (about 38%) compared to the wild type enzyme | Homo sapiens |
Y138E | the mutant shows reduced catalytic efficiency (about 15%) compared to the wild type enzyme | Homo sapiens |
Y138F | the mutant shows reduced catalytic efficiency (about 55%) compared to the wild type enzyme | Homo sapiens |
Y138K | the mutant shows severely reduced catalytic efficiency (about 5%) compared to the wild type enzyme | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.032 | - |
tetrahydrobiopterin | mutant enzyme Y138E, at pH 7.0 and 25°C | Homo sapiens | |
0.035 | - |
tetrahydrobiopterin | mutant enzyme Y138F, at pH 7.0 and 25°C | Homo sapiens | |
0.039 | - |
tetrahydrobiopterin | wild type enzyme, at pH 7.0 and 25°C | Homo sapiens | |
0.044 | - |
tetrahydrobiopterin | mutant enzyme Y138K, at pH 7.0 and 25°C | Homo sapiens | |
0.047 | - |
tetrahydrobiopterin | mutant enzyme Y138A, at pH 7.0 and 25°C | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-phenylalanine + tetrahydrobiopterin + O2 | Homo sapiens | - |
L-tyrosine + 4a-hydroxytetrahydrobiopterin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P00439 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-phenylalanine + tetrahydrobiopterin + O2 | - |
Homo sapiens | L-tyrosine + 4a-hydroxytetrahydrobiopterin | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 50000, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
PAH | - |
Homo sapiens |
phenylalanine hydroxylase | - |
Homo sapiens |