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Literature summary for 1.14.16.1 extracted from

  • Panay, A.J.; Fitzpatrick, P.F.
    Kinetic isotope effects on aromatic and benzylic hydroxylation by Chromobacterium violaceum phenylalanine hydroxylase as probes of chemical mechanism and reactivity (2008), Biochemistry, 47, 11118-11124.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.16.1

Activating Compound

Activating Compound Comment Organism Structure
dithiothreitol in the absence of dithiothreitol the activity of recently purified PheH is 50% of that achieved when dithiothreitol is present Chromobacterium violaceum

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Chromobacterium violaceum

Protein Variants

Protein Variants Comment Organism
I234D mutant shows decreased kcat value for 6,7-dimethyltetrahydropterin compared to the wild type enzyme Chromobacterium violaceum

Inhibitors

Inhibitors Comment Organism Structure
EDTA in the absence of added iron in the assay, the EDTA-treated PheH exhibits 3.5% of the L-phenylalanine hydroxylation activity of the metal-containing enzyme Chromobacterium violaceum

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ PheH is a non-heme iron monooxygenase Chromobacterium violaceum

Organism

Organism UniProt Comment Textmining
Chromobacterium violaceum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and DEAE-Sephacel gel filtration Chromobacterium violaceum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methyl-L-phenylalanine + 5,6,7,8-tetrahydrobiopterin + O2
-
 Chromobacterium violaceum ?
-
 ?
L-phenylalanine + 5,6,7,8-tetrahydrobiopterin + O2
-
 Chromobacterium violaceum L-tyrosine + 4a-hydroxytetrahydrobiopterin
-
 ?
L-phenylalanine + 6,7-dimethyltetrahydropterin + O2
-
 Chromobacterium violaceum 4-(hydroxymethyl)phenylalanine + 3-methyltyrosine + H2O + 6,7-dimethyl-dihydropterin
-
 ?
L-phenylalanine + 6-methyltetrahydropterin + O2
-
 Chromobacterium violaceum L-tyrosine + 2-amino-4a-hydroxy-7-methyl-5,6,7,8-tetrahydropteridin-4(4aH)-one
-
 ?

Synonyms

Synonyms Comment Organism
PheH
-
 Chromobacterium violaceum
phenylalanine hydroxylase
-
 Chromobacterium violaceum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.9
-
 6,7-dimethyltetrahydropterin mutant enzyme I234D, at 25°C with 50 mM HEPES (pH 7.2), 5 mM dithiothreitol Chromobacterium violaceum
1.4
-
 6,7-dimethyltetrahydropterin wild type enzyme, at 25°C with 50 mM HEPES (pH 7.2), 5 mM dithiothreitol Chromobacterium violaceum
1.6
-
 6-methyltetrahydropterin wild type enzyme, at 25°C with 50 mM HEPES (pH 7.2), 5 mM dithiothreitol Chromobacterium violaceum

Cofactor

Cofactor Comment Organism Structure
5,6,7,8-tetrahydro-L-biopterin
-
 Chromobacterium violaceum