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Literature summary for 1.14.16.1 extracted from

  • Stokka, A.J.; Flatmark, T.
    Substrate-induced conformational transition in human phenylalanine hydroxylase as studied by surface plasmon resonance analyses: the effect of terminal deletions, substrate analogues and phosphorylation (2003), Biochem. J., 369, 509-518.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.14.16.1

Protein Variants

Protein Variants Comment Organism
additional information N-terminal deletion of amino acid residues 1-102, reduction of Hill coefficient, square-wave pattern of surface plasmon resonance response. N-terminal deletion of amino acid residues 1-102 plus C-terminal deletion of amino acid residues 428-452, reduction of Hill coefficient, square-wave pattern of surface plasmon resonance response. C-terminal deletion of amino acid residues 428-452, surface plasmon resonance signal similar to wild-type Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Reaction

Reaction Comment Organism Reaction ID
L-phenylalanine + a 5,6,7,8-tetrahydropteridine + O2 = L-tyrosine + a 4a-hydroxy-5,6,7,8-tetrahydropteridine mechansim, conformational transition and hysteresis Homo sapiens
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 Hill coefficient of wild-type tetramer, substrate L-phenylalanine, 1.9, for wild-type dimer, 0.8, for N-terminal deletion mutant, 1.1, for C-terminal deletion mutant, 1.1 Homo sapiens
1.7
-
 pH 7.0, 25°C, wild-type, dimer Homo sapiens
2.22
-
 pH 7.0, 25°C, C-terminal deletion mutant Homo sapiens
4.95
-
 pH 7.0, 25°C, wild-type, tetramer Homo sapiens
6.48
-
 pH 7.0, 25°C, N-terminal deletion mutant Homo sapiens
8.32
-
 pH 7.0, 25°C, N-terminal plus C-terminal deletion mutant Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-(2-thienyl)-L-alanine + 6-methyltetrahydropterin + O2
-
 Homo sapiens ? + 6-methyldihydropterin + H2O
-
 ?
L-phenylalanine + 6-methyltetrahydropterin + O2
-
 Homo sapiens L-tyrosine + 4a-hydroxy-6-methyltetrahydropterin
-
 ?