Cloned (Comment) | Organism |
---|---|
expression of His-tagged wild-type, I65T, R261Q and V388M mutant enzymes in Escherichia coli | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
I65T | 22% of wild-type phenylalanine hydroxylase activity | Homo sapiens |
R261Q | 31% of wild-type phenylalanine hydroxylase activity | Homo sapiens |
V388M | 30% of wild-type phenylalanine hydroxylase activity | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.022 | - |
tetrahydrobiopterin | - |
Homo sapiens | |
0.082 | - |
tetrahydrobiopterin | V388M mutant enzyme | Homo sapiens | |
0.09 | - |
6-methyltetrahydropterin | - |
Homo sapiens | |
0.138 | - |
L-phenylalanine | cofactor tetrahydrobiopterin | Homo sapiens | |
0.393 | - |
L-phenylalanine | cofactor 6-methyltetrahydropterin | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type, I65T, R261Q and V388M mutant enzymes, affinity chromatography | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.055 | - |
I65T mutant enzyme, cofactor 6-methyltetrahydropterin | Homo sapiens |
0.408 | - |
V388M mutant enzyme, cofactor tetrahydrobiopterin | Homo sapiens |
0.536 | - |
R261Q mutant enzyme, cofactor tetrahydrobiopterin | Homo sapiens |
0.745 | - |
V388M mutant enzyme, cofactor 6-methyltetrahydropterin | Homo sapiens |
0.78 | - |
R261Q mutant enzyme, cofactor 6-methyltetrahydropterin | Homo sapiens |
1.742 | - |
recombinant wild-type enzyme, cofactor tetrahydrobiopterin | Homo sapiens |
2.49 | - |
recombinant wild-type enzyme, cofactor 6-methyltetrahydropterin | Homo sapiens |