Activating Compound | Comment | Organism | Structure |
---|---|---|---|
lysolecithin | 1 mM, approx. 7.5fold stimulation of phosphorylated recombinant wild-type enzyme, S16E and S16D mutant enzymes, approx. 24fold stimulation of non-phosphorylated recombinant wild-type enzyme, S16A, S16Q, S16N and S16K mutant enzymes | Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
expression of wild-type and S16E, S16Q, S16N, S16D, S16A, and S16K mutant enzyme in Escherichia coli | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
S16A | similar Km for tetrahydrobiopterin and activity as wild-type | Rattus norvegicus |
S16D | similar Km for tetrahydrobiopterin and activity as wild-type | Rattus norvegicus |
S16E | slightly higher Km for tetrahydrobiopterin than wild-type, approx. 3fold higher Vmax with phenylalanine | Rattus norvegicus |
S16K | similar Km for tetrahydrobiopterin and activity as wild-type | Rattus norvegicus |
S16N | slightly higher Km for tetrahydrobiopterin than wild-type, approx. 3fold higher Vmax with phenylalanine | Rattus norvegicus |
S16Q | slightly higher Km for tetrahydrobiopterin than wild-type, similar Vmax with phenylalanine | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0025 | - |
tetrahydrobiopterin | S16A mutant enzyme | Rattus norvegicus | |
0.0027 | - |
tetrahydrobiopterin | S16K mutant enzyme | Rattus norvegicus | |
0.0028 | - |
tetrahydrobiopterin | S16D mutant enzyme | Rattus norvegicus | |
0.0034 | - |
tetrahydrobiopterin | S16E mutant enzyme | Rattus norvegicus | |
0.0036 | - |
tetrahydrobiopterin | S16N mutant enzyme | Rattus norvegicus | |
0.0044 | - |
tetrahydrobiopterin | S16Q mutant enzyme | Rattus norvegicus | |
0.0046 | - |
tetrahydrobiopterin | phosphorylated recombinant wild-type enzyme | Rattus norvegicus | |
0.025 | - |
tetrahydrobiopterin | recombinant wild-type enzyme | Rattus norvegicus | |
0.183 | - |
phenylalanine | S16E mutant enzyme | Rattus norvegicus | |
0.187 | - |
phenylalanine | phosphorylated recombinant wild-type enzyme | Rattus norvegicus | |
0.2 | - |
phenylalanine | recombinant wild-type enzyme | Rattus norvegicus | |
0.217 | - |
phenylalanine | S16Q mutant enzyme | Rattus norvegicus | |
0.254 | - |
phenylalanine | S16K mutant enzyme | Rattus norvegicus | |
0.266 | - |
phenylalanine | S16D mutant enzyme | Rattus norvegicus | |
0.287 | - |
phenylalanine | S16N mutant enzyme | Rattus norvegicus | |
0.288 | - |
phenylalanine | S16A mutant enzyme | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
side-chain modification | phosphorylated enzyme shows a 3.3fold increase in Vmax | Rattus norvegicus |
Purification (Comment) | Organism |
---|---|
recombinant wild-type S16E, S16Q, S16N, S16D, S16A, and S16K mutant enzyme | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.9 | - |
recombinant wild-type enzyme | Rattus norvegicus |
5 | - |
phosphorylated recombinant wild-type enzyme, S16N and S16D mutant enzyme | Rattus norvegicus |
5.1 | - |
S16A and S16K mutant enzymes | Rattus norvegicus |
5.2 | - |
S16E and S16Q mutant enzyme | Rattus norvegicus |