Activating Compound | Comment | Organism | Structure |
---|---|---|---|
4-hydroxyphenylacetic acid | 0.4 mM, approx. 30% activation | Pseudomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-hydroxyphenylpyruvic acid | above 0.4 mM iron, activation below | Pseudomonas sp. | |
D,L-DOPA | 0.1 mM, approx. 60% inhibition | Pseudomonas sp. | |
D,L-m-tyrosine | 0.4 mM, approx. 80% inhibition | Pseudomonas sp. | |
dopamine | 0.5 mM, approx. 40% inhibition | Pseudomonas sp. | |
L-3,4-dihydroxyphenylalanine | - |
Pseudomonas sp. | |
L-Dopa | 0.3 mM, approx. 40% inhibition | Pseudomonas sp. | |
L-tryptophan | - |
Pseudomonas sp. | |
phenylalanine | - |
Pseudomonas sp. | |
tyrosine | - |
Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | 27000 | sucrose density gradient centrifugation, gel filtration | Pseudomonas sp. |
28000 | - |
1 * 28000, SDS-PAGE | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.9 | - |
- |
Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-phenylalanine + tetrahydrobiopterin + O2 | - |
Pseudomonas sp. | L-tyrosine + dihydrobiopterin + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 28000, SDS-PAGE | Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
tetrahydrobiopterin | - |
Pseudomonas sp. |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
phenylalanine | competitive vs. iron | Pseudomonas sp. | |
0.3 | - |
L-tryptophan | competitive vs. iron | Pseudomonas sp. | |
0.3 | - |
tyrosine | competitive vs. iron | Pseudomonas sp. |