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Literature summary for 1.14.16.1 extracted from

  • Nakata, H.; Yamauchi, T.; Fujisawa, H.
    Phenylalanine hydroxylase from Chromobacterium violaceum. Purification and characterization (1979), J. Biol. Chem., 254, 1829-1833.
    View publication on PubMed
Search for more literature for 1.14.16.1

Crystallization (Commentary)

Crystallization (Comment) Organism
vapour diffusion, 1 mg enzyme dissolved in 0.1 ml 35% ammonium sulfate, 50 mM acetate, pH 6.0, 1 mM dithiothreitol, reservoir contains 60% ammonium sulfate, crystals appear after 3-4 d at 4°C Chromobacterium violaceum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.054
-
 2-amino-4-hydroxy-6,7-dimethyltetrahydropteridine
-
 Chromobacterium violaceum
0.14
-
 L-phenylalanine
-
 Chromobacterium violaceum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000 32400 gel filtration, SDS-PAGE, sedimentation studies Chromobacterium violaceum

Organism

Organism UniProt Comment Textmining
Chromobacterium violaceum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Chromobacterium violaceum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
13.2
-
-
 Chromobacterium violaceum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-phenylalanine + 2-amino-4-hydroxy-6,7-dimethyltetrahydropteridine
-
 Chromobacterium violaceum ?
-
 r
L-phenylalanine + tetrahydrobiopterin + O2 additional electron donors: 6-methylpterin, 6,7-dimethyltetrahydropterin Chromobacterium violaceum L-tyrosine + dihydrobiopterin + H2O
-
 ?
L-phenylalanine + tetrahydrobiopterin + O2 additional electron donors: 2-amino-4-hydroxy-6,7-dimethyltetrahydropteridine, 2-amino-4-hydroxy-6-methyltetrahydropteridine, tetrahydrofolate, and 6-methyl-5-deazatetrahydropterin Chromobacterium violaceum L-tyrosine + dihydrobiopterin + H2O
-
 ?

Cofactor

Cofactor Comment Organism Structure
additional information artificial cofactors: 6-methyltetrahydropterin, 6,7-dimethyltetrahydropterin Chromobacterium violaceum
tetrahydrobiopterin
-
 Chromobacterium violaceum
tetrahydrofolate 10% of activity with 6,7-dimethyltetrahydropterin Chromobacterium violaceum