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Literature summary for 1.14.16.1 extracted from

  • Nakata, H.; Fujisawa, H.
    Purification and characterization of phenylalanine 4-monooxygenase from rat liver (1980), Biochim. Biophys. Acta, 614, 313-327.
    View publication on PubMed
Search for more literature for 1.14.16.1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0344
-
 6,7-dimethyl-5,6,7,8-tetrahydropterin enzyme form II, substrate L-phenylalanine Rattus norvegicus
0.037
-
 6-Methyl-5,6,7,8-tetrahydropterin enzyme form I, substrate L-phenylalanine Rattus norvegicus
0.0444
-
 6,7-dimethyl-5,6,7,8-tetrahydropterin enzyme form I, substrate L-phenylalanine Rattus norvegicus
0.0455
-
 6-Methyl-5,6,7,8-tetrahydropterin enzyme form II, substrate L-phenylalanine Rattus norvegicus
0.454
-
 L-phenylalanine cofactor 6-methyl-5,6,7,8-tetrahydropterin, enzyme form II Rattus norvegicus
0.72
-
 L-phenylalanine cofactor 6-methyl-5,6,7,8-tetrahydropterin, enzyme form I Rattus norvegicus
1.43
-
 L-phenylalanine cofactor 6,7-dimethyl-5,6,7,8-tetrahydropterin, enzyme form II Rattus norvegicus
1.92
-
 L-phenylalanine cofactor 6,7-dimethyl-5,6,7,8-tetrahydropterin, enzyme form I Rattus norvegicus
4.9
-
 L-tryptophan cofactor 2-amino-4-hydroxy-6-methyltetrahydropteridine Rattus norvegicus
8.5
-
 L-tryptophan cofactor 2-amino-4-hydroxy-6,7-dimethyltetrahydropteridine, enzyme form I Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Iron iron-protein Rattus norvegicus
Iron 0.6 mol of iron per mol of 51000 Da subunit Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51000
-
 4 * 51000, enzyme form I and II, SDS-PAGE Rattus norvegicus
240000
-
 sedimentation equilibrium Rattus norvegicus

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification enzyme form I contains 0.3 mol phosphate per mol subunit, enzyme form II contains 0.4 mol Rattus norvegicus

Purification (Commentary)

Purification (Comment) Organism
distinct forms I and II differing in phosphate content and isoelectric point Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.59
-
-
 Rattus norvegicus
1.46
-
-
 Rattus norvegicus
1.46
-
 enzyme form II Rattus norvegicus
1.8
-
 enzyme form I Rattus norvegicus

Storage Stability

Storage Stability Organism
-80°C, 1 month, 30% loss of activity Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-phenylalanine + tetrahydrobiopterin + O2
-
 Rattus norvegicus L-tyrosine + dihydrobiopterin + H2O
-
 ?
L-tryptophan + tetrahydrobiopterin + O2
-
 Rattus norvegicus ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 51000, enzyme form I and II, SDS-PAGE Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
9.08
-
 phenylalanine cofactor 6,7-dimethyl-5,6,7,8-tetrahydropterin Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
tetrahydrobiopterin
-
 Rattus norvegicus