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Literature summary for 1.14.15.9 extracted from
- Catalytic properties of the expressed acyclic carotenoid 2-ketolases from Rhodobacter capsulatus and Rubrivivax gelatinosus (2008), Biochim. Biophys. Acta, 1791, 125-131.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Rubrivivax gelatinosus |
| expression in Escherichia coli | Rhodobacter capsulatus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.007 | - |
spheroidene | pH 7.8, 28°C | Rubrivivax gelatinosus |  |
| 0.009 | - |
spirilloxanthin | pH 7.8, 28°C, formation of 2,2'-dioxospririlloxanthin | Rubrivivax gelatinosus | |
| 0.0101 | - |
1'-hydroxyspheroidene | pH 7.8, 28°C | Rhodobacter capsulatus | |
| 0.0136 | - |
spirilloxanthin | pH 7.8, 28°C, formation of 2,2'-diketospririlloxanthin | Rhodobacter capsulatus | |
| 0.0155 | - |
1'-hydroxyspheroidene | pH 7.8, 28°C | Rubrivivax gelatinosus | |
| 0.0169 | - |
spirilloxanthin | pH 7.8, 28°C, formation of 2-ketospririlloxanthin | Rhodobacter capsulatus | |
| 0.0181 | - |
spirilloxanthin | pH 7.8, 28°C, formation of 2-oxospririlloxanthin | Rubrivivax gelatinosus | |
| 0.0191 | - |
spheroidene | pH 7.8, 28°C | Rhodobacter capsulatus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
x * 32000, SDS-PAGE | Rhodobacter capsulatus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodobacter capsulatus | - |
- |
- |
| Rubrivivax gelatinosus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rubrivivax gelatinosus |
- |
Rhodobacter capsulatus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1'-hydroxy-1-demethylspheroidene + reduced ferredoxin + O2 | conversion rate: 28% | Rhodobacter capsulatus | 1'-hydroxy-1-demethylspheroiden-2-one + oxidized ferredoxin + H2O | - |
? | |
| 1'-hydroxy-1-demethylspheroidene + reduced ferredoxin + O2 | conversion rate: 37% | Rubrivivax gelatinosus | 1'-hydroxy-1-demethylspheroiden-2-one + oxidized ferredoxin + H2O | - |
? | |
| 1'-hydroxyspheroidene + reduced ferredoxin + O2 | conversion rate: 41% | Rhodobacter capsulatus | 1'-hydroxyspheroiden-2-one + oxidized ferredoxin + H2O | - |
? | |
| 1'-hydroxyspheroidene + reduced ferredoxin + O2 | conversion rate: 76% | Rubrivivax gelatinosus | 1'-hydroxyspheroiden-2-one + oxidized ferredoxin + H2O | - |
? | |
| 1,1'-dihydroxy-3,4-didehydrolycopene + 2 reduced ferredoxin + 2 O2 | - |
Rubrivivax gelatinosus | 2,2'-dioxo-1,1'-dihydroxy-3,4-didehydrolycopene + 2 oxidized ferredoxin + 2 H2O | - |
? | |
| 1-hydroxy-3,4-didehydrolycopene + reduced ferredoxin + O2 | - |
Rubrivivax gelatinosus | 2-oxo-1-hydroxy-3,4-didehydrolycopene + oxidized ferredoxin + H2O | - |
? | |
| 2-hydroxyspheroidene + reduced ferredoxin + O2 | - |
Rubrivivax gelatinosus | spheroiden-2-one + oxidized ferredoxin + H2O | - |
? | |
| 2-hydroxyspheroidene + reduced ferredoxin + O2 | - |
Rhodobacter capsulatus | spheroiden-2-one + oxidized ferredoxin + H2O | - |
? | |
| spheroidene + reduced ferredoxin + O2 | conversion rate: 36% | Rhodobacter capsulatus | spheroiden-2-one + oxidized ferredoxin + H2O | - |
? | |
| spheroidene + reduced ferredoxin + O2 | conversion rate: 68% | Rubrivivax gelatinosus | spheroiden-2-one + oxidized ferredoxin + H2O | - |
? | |
| spirilloxanthin + 2 reduced ferredoxin + 2 O2 | conversion rate: 41% | Rhodobacter capsulatus | 2,2'-dioxosprilloxanthin + 2 oxidized ferredoxin + 2 H2O | - |
? | |
| spirilloxanthin + 2 reduced ferredoxin + 2 O2 | conversion rate: 43% | Rubrivivax gelatinosus | 2,2'-dioxosprilloxanthin + 2 oxidized ferredoxin + 2 H2O | - |
? | |
| spirilloxanthin + reduced ferredoxin + O2 | conversion rate: 59% | Rubrivivax gelatinosus | 2-oxospirilloxanthin + oxidized ferredoxin + H2O | - |
? | |
| spirilloxanthin + reduced ferredoxin + O2 | conversion rate: 59% | Rhodobacter capsulatus | 2-oxospirilloxanthin + oxidized ferredoxin + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 32000, SDS-PAGE | Rhodobacter capsulatus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| acyclic carotenoid 2-ketolase | - |
Rubrivivax gelatinosus |
| acyclic carotenoid 2-ketolase | - |
Rhodobacter capsulatus |
| CrtA | - |
Rubrivivax gelatinosus |
| CrtA | - |
Rhodobacter capsulatus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 28 | - |
assay at | Rubrivivax gelatinosus |
| 28 | - |
assay at | Rhodobacter capsulatus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
assay at | Rubrivivax gelatinosus |
| 7.8 | - |
assay at | Rhodobacter capsulatus |
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