Literature summary for 1.14.15.8 extracted from

Schumacher, S.D.; Hannemann, F.; Teese, M.G.; Bernhardt, R.; Jose, J.
Autodisplay of functional CYP106A2 in Escherichia coli (2012), J. Biotechnol., 161, 104-112.

Search for more literature for 1.14.15.8

Cloned(Commentary)

Cloned (Comment)	Organism
functional expression of CYP106A2 in Escherichia coli strain BL21 from plasmid pET-CYP13 on the outer membrane with exposure on the surface without the external addition of the heme group but absolutely requiring the coexpression of TolC channel protein JW5503, because Escherichia coli uses a TolC-dependent mechanism to export heme into the growth media, where it can be scavenged by a surface-displayed apoenzyme	Priestia megaterium

Cloned (Comment)

Organism

functional expression of CYP106A2 in Escherichia coli strain BL21 from plasmid pET-CYP13 on the outer membrane with exposure on the surface without the external addition of the heme group but absolutely requiring the coexpression of TolC channel protein JW5503, because Escherichia coli uses a TolC-dependent mechanism to export heme into the growth media, where it can be scavenged by a surface-displayed apoenzyme

Priestia megaterium

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Fe2+	a adrenodoxin, heme, and cytochrome P450 containing enzyme	Priestia megaterium

Organism

Organism	UniProt	Comment	Textmining
Priestia megaterium	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
11-deoxycorticosterone + reduced adrenodoxin + O2	-	Priestia megaterium	15beta-hydroxy-11-deoxycorticosterone + oxidized adrenodoxin + H2O	-	?
abietic acid + reduced adrenodoxin + O2	-	Priestia megaterium	12-hydroxyabietic acid + oxidized adrenodoxin + H2O	-	?
imipramine + reduced adrenodoxin + O2	-	Priestia megaterium	desipramine + oxidized adrenodoxin + H2O	-	?
additional information	enzyme reaction is coupled to NADPH oxidation, overview	Priestia megaterium	?	-	?

Synonyms

Synonyms	Comment	Organism
CYP106A2	-	Priestia megaterium

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Priestia megaterium

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	assay at	Priestia megaterium

Cofactor

Cofactor	Comment	Organism
adrenodoxin	dependent on	Priestia megaterium
cytochrome P450	-	Priestia megaterium
heme	-	Priestia megaterium