Cloned (Comment) | Organism |
---|---|
recombinant expression of CYP106A2 by protoplast transformation is only successfully in the plasmid-less Bacillus megaterium strain MS941, not in strain ATCC 13368, coexpression of heterologous redox chain of the P450, bovine adrenodoxin reductase, and bovine adrenodoxin | Priestia megaterium |
Protein Variants | Comment | Organism |
---|---|---|
additional information | recombinant reconstitution of the whole cell conversion of 11-keto-beta-boswellic acid in Bacillus megaterium strain MS941 by coexpression of heterologous redox chain of the P450, bovine adrenodoxin reductase, and bovine adrenodoxin, as well as a a NADPH-regenerating system | Priestia megaterium |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | a cytochrome P450 enzyme | Priestia megaterium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Priestia megaterium | - |
- |
- |
Priestia megaterium ATCC 13368 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
11-oxo-beta-boswellic acid + reduced ferredoxin + O2 | a pentacyclic triterpene, 15beta-hydroxylation | Priestia megaterium | (3alpha,15beta)-3,15-dihydroxy-11-oxours-12-en-24-oic acid + H2O | - |
? | |
11-oxo-beta-boswellic acid + reduced ferredoxin + O2 | a pentacyclic triterpene, 15beta-hydroxylation | Priestia megaterium ATCC 13368 | (3alpha,15beta)-3,15-dihydroxy-11-oxours-12-en-24-oic acid + H2O | - |
? | |
abietic acid + reduced ferredoxin + O2 | a pentacyclic triterpene | Priestia megaterium | ? | - |
? | |
abietic acid + reduced ferredoxin + O2 | a pentacyclic triterpene | Priestia megaterium ATCC 13368 | ? | - |
? | |
additional information | CYP106A2 from hydroxylates a variety of 3-oxo-DELTA4 steroids and catalyzes a one-step regioselective allylic hydroxylation of the diterpene abietic acid | Priestia megaterium | ? | - |
? | |
additional information | CYP106A2 from hydroxylates a variety of 3-oxo-DELTA4 steroids and catalyzes a one-step regioselective allylic hydroxylation of the diterpene abietic acid | Priestia megaterium ATCC 13368 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CYP106A2 | - |
Priestia megaterium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Priestia megaterium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Priestia megaterium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome P450 | - |
Priestia megaterium | |
Ferredoxin | - |
Priestia megaterium |