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Literature summary for 1.14.15.7 extracted from

  • Hibino, T.; Waditee, R.; Araki, E.; Ishikawa, H.; Aoki, K.; Tanaka, Y.; Takabe, T.
    Functional characterization of choline monooxygenase, an enzyme for betaine synthesis in plants (2002), J. Biol. Chem., 277, 41352-41360.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli, Synechococcus sp. PCC7942 and Arabidopsis thaliana Spinacia oleracea

Protein Variants

Protein Variants Comment Organism
C181A second Cys in the putative consensus sequence for coordination of Rieske-type cluster to Ser, Ala and Thr, essential for activity Spinacia oleracea
C181S second Cys in the putative consensus sequence for coordination of Rieske-type cluster to Ser, Ala and Thr, essential for activity Spinacia oleracea
C181T second Cys in the putative consensus sequence for coordination of Rieske-type cluster to Ser, Ala and Thr, essential for activity Spinacia oleracea
H287A first His in the putative consensus sequence for coordination of mononuclear non-heme Fe to Gly, Ala, and Val, His-287 is essential for activity Spinacia oleracea
H287G first His in the putative consensus sequence for coordination of mononuclear non-heme Fe to Gly, Ala, and Val, His-287 is essential for activity Spinacia oleracea
H287V first His in the putative consensus sequence for coordination of mononuclear non-heme Fe to Gly, Ala, and Val, His-287 is essential for activity Spinacia oleracea

Metals/Ions

Metals/Ions Comment Organism Structure
Iron enzyme contains a Rieske-type iron-sulfur center Spinacia oleracea

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
choline + O2 + reduced ferredoxin Spinacia oleracea
-
betaine aldehyde hydrate + H2O + oxidized ferredoxin
-
?

Organism

Organism UniProt Comment Textmining
Spinacia oleracea O04121
-
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Spinacia oleracea
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
choline + O2 + reduced ferredoxin
-
Spinacia oleracea betaine aldehyde hydrate + H2O + oxidized ferredoxin
-
?

Synonyms

Synonyms Comment Organism
CMO
-
Spinacia oleracea